群体感应（QS）介导内生Burkholderia Pyrrocinia的生防分子机制

群体感应（QS）在细菌的许多生物学功能中发挥重要作用。然而，QS系统在内生生防细菌中的功能目前尚不明确。吡咯伯克霍尔德氏菌JK-SH007是一株兼具促生和高效防治杨树溃疡病的内生细菌。本项目拟在前期研究的基础上，围绕JK-SH007菌株QS系统的关键基因，探索该菌株的群体感应系统及信号物质；构建QS系统关键基因敲除体，采用转录组测序及分析技术研究获得QS关键基因调控的促生长、抗病性相关下游基因，并通过基因敲除及过表达技术解析基因功能；构建出该菌株的QS通讯网络系统，并探索菌株QS在杨树溃疡病防治中的作用，以期在分子水平上揭示JK-SH007菌株对杨树的抗病促生机制，为杨树病害的综合防治开辟新的途径。研究结果将进一步完善内生生防菌的生防分子机制，将为充分利用植物内生菌资源，提高杨树病害的生物防治能力奠定理论基础和提供技术支撑。

Quorum sensing (QS) plays an important role in many biological functions of bacteria. However, so far very little is known about the roles of the QS system in the endophytic biocontrol bacteria. Here, the high-efficient endophytic antagonistic bacteria Burkholderia pyrrocinia JK-SH007 with biocontrol activity against poplar canker and growth promoting characteristic will be advantaged. Firstly, the kinds and numbers of QS system and signal substances of strain JK-SH007 will be studied. Secondly, the key-gene knockout mutants will be constructed. Thirdly, downstream genes associated with growth promotion and disease resistance will be obtained through transcriptome resequencing and bioinformation analysis technology and make functional verification through gene knockout and overexpression techniques. Then, the QS communication network system of strain JK-SH007 will be constructed and the effect of strain QS on the control of poplar canker will be studied. The results of this study will further improve the biocontrol molecular mechanism of endogenous bacteria control, and lay a theoretical foundation and provide technical support for making full use of endophytic bacteria resources to improve the biological control ability of poplar diseases.