“治神调髓”针刺调控Nrf2/ARE介导少突胶质细胞分化在PISD脑白质损伤中的作用机制研究

当前，缺血性卒中后抑郁（Post ischemic stroke depression，PISD）已成为一个日益严重的公共卫生问题。缺血性卒中脑白质损伤过程中的氧化应激反应是一个关键环节，而Nrf2/ARE通路及其介导的一系列自由基清除酶类在氧化应激过程中发挥着关键作用。课题组前期研究结果表明治神调髓针刺能够通过抗氧化应激、抗炎性反应改善PISD脑白质损伤，然而具体机制尚待明了。本课题拟从Nrf2/ARE通路着手，以少突胶质细胞分化为切入点，采用细胞形态学、免疫组化学、分子生物学等技术，通过离体和在体研究相结合，深入探讨治神调髓针刺对Nrf2/ARE通路的调控作用，及其对脑白质损伤的保护作用机制，在进一步丰富“治神调髓”理论科学内涵的同时，以期为针灸治疗PISD的临床应用提供新思路，从而达到一矢双穿的效果。

Nowadays,post ischemic stroke depression has become an increasingly serious public health problem.Oxidative stress is a key link in the process of white matter injury in ischemic stroke,Nrf2/ARE signaling pathway and a series of free radical scavengers mediated by the pathway play a key role in oxidative stress.Preliminary studies by the research group showed that the acupuncture method could improve PISD white matter injury through anti-oxidative stress and anti-inflammatory response, but the mechanism is still unknown.In this stuy,we would take Nrf2/ARE signaling pathway and oligodendrocyte differentiation as an entry point,by means of Cell morphology, immunohistochemistry, molecular biology,through vitro and vivo studies to investigate the regulation mechanism of Zhishen Tiaosui acupuncture on Nrf2/ARE signaling pathway and the protection mechanism of it on injured white matter.The aim of this study is to enrich the scientific connotation of the 'Zhishen Tiaosui'theory and to provide a new ideas for clinical application of acupuncture treating PISD.