单组份双生病毒调控作物抗虫性的分子机制

植物病毒侵染可以调控作物的抗虫性，进而影响昆虫的种群动态及与其伴随的多种作物病害，因此，病毒对作物抗虫性的调控可能是影响田间昆虫及其伴随病害的重要因素之一。对于双生病毒而言，之前相关的研究大都集中在病毒伴随卫星对作物抗虫性的调控上，而对于无伴随卫星的单组分双生病毒对作物抗虫性的调控以及昆虫对抗虫性变化的响应机制，则基本没有报道。在本项目中，我们在前期发现番茄黄曲叶病毒可能通过其C2蛋白与多个烟草蛋白的互作实现对烟草抗虫性调控的基础上，计划通过对多个无伴随卫星单组份双生病毒-作物-烟粉虱组合的研究，从病毒和作物两个角度出发进一步明确无伴随卫星单组份双生病毒对作物抗虫性的调控及机制，同时从烟粉虱的角度出发探究昆虫对这种抗虫性变化的响应机制。本研究不仅有助于解析病毒对作物的侵染和调控过程，而且有助于深入理解作物的抗虫防御机制，为抗病抗虫育种提供新的理论基础。

The infection of plant viruses in crops can have significant impacts on the resistance to insects, and by doing so the population dynamics of insect and insect-borne plant pathogens are greatly affected. Though important, the manipulation of plant defense by many plant viruses such as geminiviruses have been characterized in a few cases but examined rarely especially at the physiological and molecular levels. For manipulation of plant defense by geminiviruses, previous researches focus on the role of their associated satellites, whereas little is known with regard to the manipulation of plant defense by the geminiviruses those did not have associated satellites. And the response of insects, such as whiteflies, to the manipulation of plant defense by plant viruses remains poorly understood. Here in the present project, based on preliminary findings showing that Tomato leaf curl yellow virus might regulate the resistance of Nicotiana tabacum to whitefly through the interaction between C2 protein and multiple plant proteins, we plan to conduct a serial of analysis using multiple combinations of geminivirus (those without satellite) -crop-whitefly species to identify the key factors in geminiviruses and crops those determine the manipulation of defense and elucidate the underlying molecular mechanism. Also, we aim to decipher the key mechanism involved in the response of whitefly to the manipulation of crop defense by geminiviruses. The successful implementation of this project will not only shed light on the infection and manipulation of crops by plant viruses, but also contribute to a better understanding of the nature of plant defense to insect pests and the development of novel crop varieties.