青藏高原人工草地恢复过程中丛枝菌根真菌群落演化机制研究

青藏高原是世界上最大的高寒草地生态系统分布区，对气候环境变化较为敏感。由于全球气候变化、人为干扰等原因，青藏高原高寒草地生态系统退化日益严重，建植人工草地成为解决退化问题的主要途径之一。丛枝菌根真菌（AMF）在改善土壤环境、促进养分吸收、帮助生态恢复等方面起重要作用。然而，目前关于AMF对人工建植草地恢复程度响应的研究较少。本研究将天然未退化的草地作为对照，以不同建植年限的人工草地为研究对象，探讨在草地恢复过程中AMF生物量及多样性的变化规律及形成机制；通过传统生物学方法与现代分子生物学方法的结合，解析不同环境因子对AMF各生物学属性的作用机制；利用Mantel检验、结构方程模型以及多元逐步回归等统计学方法，厘清植被恢复与AMF生物量、群落结构及功能活性的关系。本项目研究结果为AMF在植被恢复过程中的应用奠定基础，同时为高寒草地生态系统科学管理及生物多样性保护提供了一定的理论依据。

Qinghai-Tibetan Plateau has the largest alpine grassland ecosystem in the world, which is sensitive to climate and environment changes. Due to natural and anthropogenic disturbances, the degradation of alpine grassland ecosystem on Qinghai-Tibetan Plateau becomes a serious problem. And the main restoration practices are artificial revegetation of the degraded grasslands. It has been demonstrated that arbuscular mycorrhizal fungi (AMF) play important roles in improving soil environment, promoting plant nutrient uptake, and helping ecological restoration. However, investigations on the response of AMF grassland restoration in Qinghai-Tibetan Plateau remain unclear. This project focuses on natural grassland and artificial grassland with different establishment years, explores the dynamics of AMF community structure, biomass and activity in the process of grassland restoration. By the combination of traditional biological methods and modern molecular methods, the effects of different environmental factors on AMF biological traits are analyzed. Using Mantel test, structural equation modeling and multiple stepwise regression, the relationship between vegetation restoration and AMF biomass, community structure and activity are clarified. The results of this study are expected lay the foundation for the application of AMF in revegetation practices, and provide theoretical basis for the management of alpine grassland ecosystems and biodiversity conservation.