水稻受体激酶与膜支架蛋白CPR5的互作和功能研究

单次跨膜受体激酶（RLK）是植物中最大的受体蛋白家族，RLK的工作原理及遗传功能是重大的基础科学问题。目前对RLK的研究多集中于其细胞膜外配体信号感受和膜内信号传递机制上，但对其如何调控膜上相关复合体的组装等事件所知甚少。课题组发现受体激酶FLR家族在水稻中调控育性、株高、稻米品质和病菌响应等过程；其家族成员FLR2与多次跨膜支架蛋白CPR5互作，调控稻瘟菌应答。基于此，以水稻为材料，拟（1）确定FLR2与CPR5互作的亚细胞位置、磷酸化等生化机理；（2）阐明CPR5表达模式和遗传功能，以及FLR2-CRP5互作所调控的农艺性状;（3）以稻瘟菌为模型，初步探究脂质微区定位的FLR2-CPR5支架如何调节膜上相关蛋白复合体组装，响应稻瘟菌。力图建立受体激酶通过多次跨膜蛋白调控膜上相关复合体组装事件，从而影响植物环境适应性的模型，为优良农作物品种的分子育种设计提供新靶标。

Single-pass transmembrane receptor kinases (RLK) are the largest receptor family in plants. Their genetic function and underlying mechanism are fundamental but largely unclear. At present, most of studies on RLK focus on extracellular ligand perception and intracellular signal transduction. Little is known about how RLK regulate the assembly of their related complex on the plasma membrane. We found that in rice, receptor kinase FLR family could regulate fertility, plant height, nutrient quality, reponse to fungus; a member of this family, FLR2, interacted with a multiple transmembrane scaffold protein CPR5 and triggered the innate immunity to pathogen Magnaporthe grisea. Based on these results, we will study (1) the cellular and biochemical mechanism of the interaction between FLR2 and CPR5, such as subcellular localization and phosphorylation; (2) the expression pattern and genetic function of CPR5 and the agronomic traits driven by FLR2-CRP5 interaction; (3) the formation of lipid-microdomain-localizing FLR2-CPR5 complex in response to a model pathogen Magnaporthe grisea. This project will set up a model on RLK regulating the assembly of their related complex through a multiple transmembrane protein and thus affecting plant environmental adaptability and will provide putative targets for molecular breeding design of excellent crop varieties.