OsABCC2调控噻虫嗪在水稻茎节和籽粒间分配的功能研究

借助农药转运蛋白实现农药在作物中的定向积累，是提高农药对靶传递效率和减少农残的重要途径。水稻茎节是控制物质运输分配的枢纽，分布于茎节的转运蛋白决定着输送的物质滞留茎叶或者流向稻穗。项目前期通过筛选富集表达1452个水稻膜蛋白的酵母文库，获得噻虫嗪转运基因OsABCC2，属水稻ATP结合蛋白C家族。该家族有些成员能够在茎节部位高表达, 具有转运叶酸及有机分子的功能。敲除和过表达OsABCC2显著改变了植株对噻虫嗪的吸收和转运，但其具体规律和机制不明确。本项目拟从植株、细胞和分子互作等层面，通过免疫染色定位、高分辨率质谱成像、韧皮部特异表达植株靶向积累噻虫嗪杀虫药效测定、转运动力学分析等方法，研究OsABCC2调控噻虫嗪在水稻茎节和籽粒分配的功能，明确OsABCC2蛋白与噻虫嗪分子互作的活性位点。项目可望为构建农药施用“智能轻简”作物，实现农药害虫为害部位定向积累的实践提供信息和依据。

Accumulation of pesticides at the site of pest damaged in crops with the help of pesticide transporters is one of the effective ways to improve the efficiency of pesticide-target-delivery in crops and to reduce agricultural chemical residues. Node in rice plant is a hub to control substrate transportation and distribution. Membrane proteins in the node of rice determines the way that substrates transported to stems, or leaves, or panicles. In previous study, a thiamethoxam transporter gene OsABCC2 was screened from a yeast library enriched expressing of 1452 membrane proteins. OsABCC2 belongs to rice ATP-binding cassette (ABC) type C family. Members of ABCC family could highly express in node and transfer folic acid and organic molecular etc. We had shown that knockout or overexpression of OsABCC2 significantly changed the translocation and distribution of thiamethoxam in rice, but its mechanism needs to be further explored. In this project, functions of OsABCC2 involved in thiamethoxam translocation and distribution between the node and grain will be revealed in plant, cell, as well as molecular level. Experiments such as immunostaining localization, high resolution mass spectrometry imaging, measurement of thiamethoxam control efficacy to rice planthopper in phloem-specific-expression plants, thiamethoxam transport dynamics analysis will be carried out. The implementation of the project is expected to provide a reference for construct of ‘easy’ crops, which are able to accumulate pesticide to the pest damage sites by the involvement of transporters.