整合素连接激酶在人脐带间充质干细胞中的过度表达治疗放射性溃疡的实验研究

放射性皮肤溃疡是由恶性肿瘤的治疗、核事故等引起，由于反复复发而给患者带来巨大痛苦。目前以皮瓣移植、高压氧疗、VSD负压封闭吸引等治疗为主，难治和复发率高。近年，细胞治疗作为一种新的治疗方法，也应用于慢性创面的修复，而脐带来源的间充质干细胞（hUCMSCs）取材方便，无伦理学争议，成为修复的主要种子细胞。但hUCMSCs移植后存活率的下降，极大地限制了细胞治疗的有效性。如何提高细胞移植中hUCMSCs的存活率？利用基因工程改善间充质干细胞的功能可能是提高干细胞效率的一种可行的方法。整合素连接激酶（Integrin linked kinase，ILK）是一种丝氨酸-苏氨酸蛋白激酶，参与调节细胞增殖、分化、存活和凋亡、血管生成等。本课题拟采用整合素连接激酶在脐带间充质干细胞中过表达，修复放射性溃疡，通过体内外实验观察治疗作用并深入探讨治疗机制，为开发新的慢性创面治疗技术和药物提供理论依据

Radiation skin ulcer is caused by the treatment of malignant tumors, nuclear accidents and so on, which brings great pain to patients due to repeated recurrence. At present, skin flap transplantation, hyperbaric oxygen therapy, VSD vacuum sealing and suction are the main treatment, which is difficult to treat and has a high recurrence rate. In recent years, as a new treatment method, cell therapy has also been applied to the repair of chronic wounds. However, the cord derived mesenchymal stem cells (ucmscs) are easy to obtain and have no ethical dispute, and become the main seed cells for repair. However, the decline of survival rate after transplantation of hUCMSCs greatly limits the effectiveness of cell therapy. How to improve the survival rate of hUCMSCs in cell transplantation? Improving the function of mesenchymal stem cells by genetic engineering may be a feasible way to improve the efficiency of stem cells. Integrin linked kinase (ILK) is a serine threonine protein kinase, which is involved in the regulation of cell proliferation, differentiation, survival and apoptosis, angiogenesis and so on. In this study, integrin linked kinase was overexpressed in umbilical cord mesenchymal stem cells to repair radiation ulcer. The therapeutic effect was observed in vitro and in vivo, and the therapeutic mechanism was discussed in depth, which provided theoretical basis for the development of new chronic wound treatment technology and drugs.