CW光激发的ICG近红外反斯托克斯荧光特性及其活体功能应用

反斯托克斯发光生物成像具有信噪比高、深度大和光损伤小等众多优势。基于热带吸收的反斯托克斯发光可通过CW激光激发实现，且显著依赖于温度，已被逐渐用于生物成像、温度传感等。然而，由于缺乏激发和荧光发射波长都位于近红外波段、且具有高生物兼容性、可代谢的小分子探针，基于热带吸收的反斯托克斯发光成像很少被用于活体研究。在本项目中，我们拟对唯一在临床上使用的近红外荧光成像造影剂ICG的热带吸收诱导反斯托克斯发光特性进行系统研究，搭建反斯托克斯荧光小动物全身成像系统、扫描显微成像系统等，开展ICG在活体功能成像、光热治疗、生物调控中的应用，如：小动物全身成像、脑血管扫描显微成像、活体光热治疗过程中温度的实时监测、光致制冷生物调控等。为材料化学研究者们开发新的近红外热带吸收材料提供借鉴，为将来的基础医学研究和潜在的临床应用提供参考。

Anti-stokes luminescence bioimaging possesses the advantages of high signal to noise ratio, large penetration depth and low photon-damage. Anti-stokes luminescence based on hot-band absorption can be directly excited by the continuous wave (CW) laser and is highly dependent on the temperature, and has been gradually employed for bioimaging, temperature sensing and so on. However, due to the lack of biocompatible and clearable small-molecule probes whose excitation and emission wavelength are both in the near infrared (NIR) spectral region, optical bioimaging based on hot-band absorption induced anti-stokes luminescence are rarely utilized for in vivo study. In this proposed project, we will systematically investigate the hot-band absorption induced anti-stokes luminescence features of indocyanine green (ICG), which is the only kind of NIR optical contrast agent adopted in clinically applications. We will further build anti-stokes fluorescence in vivo whole-body and scanning microscopic imaging systems, and utilize ICG for in vivo functional imaging, photothermal therapy, and biological regulating, including small animal whole-body organ imaging, real-time temperature monitoring during in vivo photothermal therapy, photon-induced cooling for biological stimulation, and so on. We really hope that our work can provide references for developing new NIR-emitted hot-band absorption materials, as well as be helpful for the future basic medical research and potential clinical applications.