分子伴侣蛋白SAJ1与EDS1互作调控植物抗病防御的机理研究

植物病害严重危害农业生产和影响食品安全，植物为了生存进化出精细而复杂的调控机制应对病虫害的攻击。活体营养型病原菌侵染植株时，免疫正调控因子EDS1表达上调，促进抗性激素SA的积累，进而激活下游防御应答信号转导和基因表达。很多研究表明EDS1在亚细胞水平的核质分布对抗病应答具有重要的作用。然而，在这个过程中，EDS1如何在亚细胞水平合理的分配尚不清楚。我们研究发现一个定位在叶绿体的分子伴侣蛋白SAJ1参与EDS1介导的抗病防御应答，并与EDS1在细胞质互作，提示SAJ1可能通过调控EDS1的核质分配进而在抗病应答中发挥作用。在此基础上，本项目拟通过深入研究SAJ1与EDS1互作发生的机理和对EDS1抗病性的影响，探知SAJ1如何在亚细胞水平调控EDS1，以阐明SAJ1负调控EDS1进而调控植物抗病防御响应的分子机制，为增强对植物免疫机理的深入理解，并将其应用于作物抗病育种提供良好的理论基础。

Plant diseases seriously harm agricultural production and affect food safety. Plants have evolved elaborate and complex regulatory mechanisms for survival in response to plant pests and diseases. It is very important to reveal the regulatory mechanism of plant defense response against disease. When biotrophic pathogens infect Arabidopsis, the expression of positive immune regulator EDS1 is up-regulated, which promotes the accumulation of resistance hormone SA, and then activates downstream defense response signal transduction and gene expression. Many studies have shown that the nucleo-cytoplasmic distribution of EDS1 at the subcellular level plays an important role in disease response. However, it is not clear how EDS1 is properly distributed at the subcellular level during this process. Our previous study found that a molecular chaperone SAJ1 located in the chloroplast is involved in the EDS1 mediated disease resistance defense response and interacts with the cytoplasmic EDS1 generating protein, suggesting that SAJ1 may play a role in disease resistance response by regulating the nucleo-cytoplasmic trafficking of EDS1. On this basis, this project intends to through the study of mechanism of SAJ1 and EDS1 interactions and the impact on the EDS1 disease resistance, ascertain how SAJ1 regulated EDS1 in subcellular level, to clarify SAJ1 through negative regulation EDS1 to participate in the molecular mechanism of plant resistance defense response, to enhance understanding of the plant immune mechanism and applied to crop disease resistance breeding to provide a good theoretical basis.