基于耗散结构的T/P-SAEW并联加工对E.coli协同杀菌效应熵驱动机制的动力学研究

如何在确保杀菌效果的同时，有效降低杀菌处理对食品可能的负面影响？针对这一问题，项目组提出：通过技术组合，在杀菌效果靶向增强的同时，实现负面影响的多靶点分散削弱。前期工作中围绕微酸性电解水与热杀菌或高压杀菌的并联组合，发现了“1+1>2”模式的协同杀菌效应(SSE)，并提出SSE的熵驱动假说。本项目将围绕SSE熵驱动机制这一科学问题，以代谢网络信息完整的E.coli为对象，通过对杀菌过程的表观动力学分析，建立SSE发生的动力学判据；进而基于耗散结构理论，构建胞内物质流网络攻击模型，探讨介观个体杀灭过程与表观群体杀菌过程的统计关系，据此建立杀菌过程的熵驱动理论；然后通过对不同技术所引发热力学流与热力学力的耦合作用分析，揭示SSE的熵驱动机制；最后基于E.coli代谢网络，利用神经网络模拟，探讨熵增率的节点分布及其时间演化，阐明SSE的生物学基础。相关成果为新型绿色杀菌技术的开发提供理论支撑。

The single sterilization technique can not only enhance its sterilization effect, but also increase the negative effect on food. In response to this problem, the project team proposed a combination of technologies to achieve targeted enhancement of the sterilization effect while controlling negative effects. In the previous work, the project team found the phenomenon of synergistic sterilization effect which satisfies the "1+1>2" mode under the parallel processing of slightly acidic electrolyzed water with heating or pressurization, and hypothesized that the synergistic sterilization effect is the result of entropy driven. This project will study on this scientific problem of entropy driving mechanism of synergistic sterilization effect. Firstly, study on the apparent kinetics of sterilization process and establish the kinetic criterion of synergistic sterilization effect. And then, based on the dissipative structure model, the statistical relationship between mesoscopic individual sterilization process and apparent population sterilization process was explored, to establish the entropy driving theory of sterilization process. And through the coupling analysis of thermodynamic flow and thermodynamic force caused by different technologies, the entropy driving mechanism of synergistic sterilization effect is revealed. Finally, based on E.coli metabolic network, artificial neural network simulation is used to explore the network node distribution and time evolution of entropy increase rate, and clarify the biological basis of synergistic sterilization effect. The results have guiding significance for the development of new green sterilization technology.