肠道菌群失调在小麦依赖-运动诱发严重过敏反应发病中的作用与机制研究

小麦依赖-运动诱发严重过敏反应（WDEIA）是一种特殊类型的食物过敏，发病机制未明，缺乏有效治疗手段，易出现危及生命的严重过敏反应。目前已有研究证明肠道菌群及其代谢产物在食物过敏的发病中起重要的作用，而其在WDEIA的发病中是否发挥作用尚无报道。本课题组在前期研究中，发现肠道菌群可能与WDEIA的发病相关。本课题旨在研究肠道菌群及其代谢物在WDEIA发病中的作用机制。本课题将在前期研究的基础上，应用宏基因组学技术检测WDEIA患者粪便中的菌群，验证肠道菌群在WDEIA发病中的重要作用。应用代谢组学方法，确定肠道菌群是否通过其代谢产物影响WDEIA的发生，研究与WDEIA发病相关的代谢通路，再通过分析肠道菌群及其代谢产物与免疫细胞的关系，进一步阐明WDEIA的发病机制，以期通过肠道菌群及代谢物的研究，为WDEIA的治疗提供新的方向。

Wheat-dependent exercise-induced anaphylaxis (WDEIA) is a special type of food allergy. The WDEIA etiology remains elusive, no effective treatment is currently available and patients are prone to life-threatening anaphylaxis. Increasing evidence shows that intestinal microbiota and its metabolites play an important role in the development of food allergy, but whether they play a role in the pathogenesis of WDEIA have not been reported. In our previous study, we identified a potential association between the gut microbiome and WDEIA development. This project aims to study the role and mechanism of gut microbiome and its metabolites in WDEIA. Based on our previous study, this project will use metagenomics technology to detect the microbiota in the fecal samples of WDEIA patients and verify the important role of intestinal microbiota in the pathogenesis of WDEIA, apply metabolomics to determine whether the intestinal microbiota affects the onset of WDEIA through its metabolites, explore the metabolic pathways related to the pathogenesis of WDEIA, analyze the relationship between the intestinal microbiome, its metabolites and immune cells, and further clarify the pathogenesis of WDEIA. This project aims to provide a new direction for the treatment of WDEIA through the research of intestinal microbiota and metabolites.