大豆驯化基因GmPRR3b调控主茎节数的机制研究

大豆果荚生长附着在茎节上，因此有效茎节数是决定大豆产量的关键性状之一，在保证主茎直立前提下增加有效茎节数是选育理想株型高产大豆新品种的重要目标。由于直接控制大豆主茎节数的基因和作用机制还很不清楚，限制了大豆理想株型的分子选育。申请人前期通过全基因组关联分析，克隆了控制大豆生育期的关键驯化基因GmPRR3b，并揭示了其介导光周期和生物钟调控大豆开花的分子机制；GmPRR3b基因单倍型H6具有促进大豆开花和加速茎节生长的双重效应，因此在大豆驯化和遗传改良过程中受到强烈选择。在此基础上，本研究将通过多种技术手段鉴定GmPRR3b的互作蛋白和下游基因，解析GmPRR3b单倍型H6增加茎节数的分子机制，为培育理想株型大豆新品种提供理论支撑和新材料。

The number of effective stem nodes that carry pods is one of the key factors determining soybean yield potential. It has been proposed that a high yield soybean cultivar of ideal plant architecture (IPA) should be characterized with erect stems with more effective nodes. Currently, the genetic loci and action mechanisms that directly control the number of main stem nodes in soybean are unclear yet, which significantly delays the molecular breeding of IPA soybean cultivars. Previously, we identified a key domestication gene GmPRR3b, which controls soybean growth period, through genome-wide association analysis (GWAS). We found that the GmPRR3b haplotype H6 had been exclusively selected during domestication and genetic improvement, possibly due to its beneficial effects in both promoting flora transition and node growth. In this study, we plan to identify the GmPRR3b interaction proteins and downstream genes, and dissect the molecular mechanisms of how GmPRR3b haploid H6 accelerates node growth. This work will provide theoretical guidance and breeding materials for the breeding of IPA soybean varieties in future.