行气逐水法调控GSH/ROS/Ferroptosis系统干预膀胱癌作用及分子机制研究

膀胱癌是泌尿系统最常见的恶性肿瘤之一，其易复发的特点已成为临床治疗难点。课题组基于膀胱癌患者“膀胱气化失司，水湿不化，湿热蕴结于膀胱”的病机特点，运用“行气逐水法”治疗膀胱癌，能有效降低膀胱癌术后复发率，改善生活质量。我们前期体内外实验研究也发现，行气逐水法能通过GSH/ROS/Ferroptosis系统抑制膀胱癌细胞，且能调控铁死亡通路关键基因表达。故我们提出“行气逐水法通过调控铁死亡通路相关基因的表达诱导膀胱癌细胞内ROS水平增高，促使膀胱癌细胞发生铁死亡”的科学假说。本研究拟通过原位瘤动物模型实验进一步验证行气逐水中药在体内的抗癌作用，并通过基因表达谱芯片、细胞生物学、分子生物学、构建高低表达GPX4细胞模型等手段，阐明“行气逐水法”通过调控铁死亡相关通路治疗膀胱癌的分子生物学机制。上述研究有望为“行气逐水法”治疗膀胱癌提供科学依据。

Bladder cancer is one of the most common malignant tumors of the urinary system,but its easy recurrence has become a clinical difficulty.We treat the bladder cancer with the method“Promoting qi and expelling retained fluid affect”,which is based on the pathogenesis“the bladder lose the function of promoting qi,the fluid doesn't flow,the heat and damp stay in bladder”.The treatment can reduce the recurrence rate of bladder cancer and improve the quality of life.Through previous experiments in vivo and in vitro,we discovered that the method of promoting qi and expelling retained fluid inhibit the bladder cancer cell through GSH/ROS/Ferroptosis system and regulate the key genes of the system. Therefore,we scientifically hypothesized that the method of promoting qi and expelling retained fluid can increase the level of ros by regulating the genes of ferroptosis system,which can cause ferroptosis.In this study,we first plan to verify the anticancer effect of promoting qi and expelling retained fluid in ovarian cancer model.Then,gene expression profile chipcell biology methods, molecular biology methods、Construction higher and lower expression of GPX4 cell model are used to reveal the mechanism of“promoting qi and expelling retained fluid”in the treatment of bladder cancer by regulating ferroptosis related pathway.The above study may provide a scientific basis for the treatment of bladder cancer with the method of“promoting qi and expelling retained fluid”.