受体激酶FERONIA调控植物根再生的机理研究

植物具有强大的再生能力以适应严苛的自然环境，这种能力已被广泛运用至农业生产，比如扦插、组织培养等。再生的本质是伤口或胁迫信号指导的细胞命运重编程，然而我们对其细胞学和分子机理的认识还十分欠缺。本项目前期的正向遗传学筛选表明受体激酶FERONIA参与根器官再生调控，其功能缺失突变体的根创始细胞无法正常分裂成根原基。经回复子筛选，我们定位到一个MAPKKK家族基因SOF225（SUPPRESSOR OF FERONIA 225）。初步研究表明再生过程中该基因高表达于根创始细胞，其低水平表达株表现出和fer相同的根再生缺陷，且在酵母中与FER的胞内域互作。我们推测其作为FER的下游调控根创始细胞的分裂以建立根原基。本项目将综合运用遗传学、生化及细胞学手段来揭示FER通过SOF225调控再生的细胞学和分子基础。这将帮助我们认识复杂而强大的再生现象背后的规律，同时在指导农业和园艺应用上具有重要意义。

Plants have evolved powerful regeneration ability to survive rigorous environment, which has been widely exploited in agricultural technologies such as cuttage and tissue culture for vegetative propagation. Regeneration is a process of the cell fate reprogramming upon wounding or stress. However the underlying mechanisms are poorly understood. Our previous genetic screen revealed that receptor kinase FERONIA was involved in root regeneration, its loss-of-function mutant could not establish root primordium from root founder cell division. Through suppressor screening, we identified a MAPKKK gene, SUPPRESSOR OF FERONIA 225 (SOF225). Preliminary studies showed SOF225 was highly expressed in root founder cells during root regeneration, and leaf explants from SOF225 knock down lines exhibited similar defects in root regeneration as in fer mutants. We propose that SOF225 may act downstream of FER to regulate root founder cells division to establish root primordium. In this project, genetics, biochemistry and cytology evidences would be provided to reveal the cellular and molecular basis of FER and SOF225 in root regeneration. This will help us to understand the powerful regulation pattern behind regeneration, and will also have important practical value in guiding the technology application in agriculture and horticulture.