JAZ3对番茄叶霉病抗病基因Cfs所介导免疫应答体系的调控机制研究及应用探索

叶霉病对番茄生产影响严重。Cfs基因可以介导叶霉病垂直抗性，但由于不同家族成员应答机制存在差异性，且可供研究的已克隆Cf基因较少，所以Cfs抗病体系调控机制一直难以整合；同时由于病原菌分化迅速，育种中面临抗源匮乏的困境。团队前期研究发现在不同Cf基因激活应答后，JAZ3下调表达或敲除可以增强超敏反应，提高植株抗性，说明JAZ3调控模块是不同Cf基因下游应答共有部分，可以为机制整合研究提供新的切入点，另外由于JAZ3可以在不同Cf基因下游发挥负调控作用，为利用现有抗源解决育种抗性需求提供了可能。因此本研究将利用不同抗性等级Cf抗源材料，构建JAZ3超表达和基因编辑敲除株系，通过转录组对比、JAZ3互作蛋白筛选、重要相关基因和蛋白功能分析等手段解析JAZ3对Cfs抗病应答体系的调控机制；同时在携带不同Cf抗源的核心种质材料中敲除JAZ3基因，评价改良效果，为突破Cf抗源匮乏的育种瓶颈提供契机。

Tomato leaf mold affects tomato production seriously. Cf genes can mediate vertical resistance to leaf mold. However, due to the complexity and diversity of response mechanisms of different family members, and the few cloned Cf genes available for research, the regulatory mechanism of Cf resistance system has been difficult to integrate. At the same time, due to the rapid differentiation of pathogen, the breeding also faces the dilemma of resistance gene shortage. Previous studies have found that the down-regulation or knockout of JAZ3 can enhance the hypersensitivity response and improve the plant resistance in the case of resistance response activated by different Cf genes, indicating that JAZ3 regulatory module is a common part of different Cf downstream responses, which can provide an excellent entry point for mechanism integration research. In addition, because the negative regulatory effect of JAZ3 can play a role in the downstream of different Cf genes, it is possible to use the existing Cf genes to meet the needs of resistance in breeding. Therefore, in this study, we will construct JAZ3 overexpression and gene editing knock-out lines using Cf-carried materials with different resistance levels, and analyze the regulatory mechanism of JAZ3 on Cf-mediated disease resistance response system by means of transcriptome comparison, JAZ3 interaction protein screening, function and regulatory relationship analysis of important related genes and proteins; At the same time, JAZ3 gene will be knocked out in the core germplasm materials carrying different Cf genes to evaluate the improvement effect, so as to explore a way to break through the breeding bottleneck of lack of Cf gene.