抗污染核酸适配体微电极的构筑及其活体电化学分析应用

活体层次神经化学物质的精准测量对于深入了解脑功能以及神经系统疾病发生和发展的分子机制具有重要意义。本项目拟针对活体电化学分析中电极选择性及稳定性等关键科学问题，通过发展电极界面组装新原理，构筑兼具高选择性、强抗蛋白吸附以及生物兼容的电极界面，为在活体层次开展神经化学物质如多巴胺、5-羟色胺的高选择性活体电化学分析提供重要的分析工具。具体包括以下三方面工作：1）构筑核酸适配体-细胞膜修饰微电极体系；2）考察核酸适配体-细胞膜修饰电极的电分析化学性能；3）考察核酸适配体-细胞膜修饰微电极的活体分析应用。本项目的发展，将为研究脑功能及神经系统疾病发生发展的分子机制提供重要的分析工具。

Developing in vivo analytical tools to realize the precise determination and capturing the dynamics of neurochemicals is of great significance to unraveling the chemical essence of brain function and understanding the molecular mechanisms of many pathological processes. To improve the selectivity and stability of current electrochemical platform, this proposal aims to designing new interfacial chemistry for constructing a highly selective and antibiofouling electrochemical interface for in vivo analysis of neurochemicals, including dopamine and 5-hydroxytryptamine. Specifically, this proposal will carry out the following three specific aims: (1) Design new strategies to construct aptamer-cell membrane modified carbon fiber microelectrode(CFE); (2) Evaluate the analytical performance of modified CFE in detection of dopamine and 5-hydroxytryptamine; (3) Evaluate the in vivo application of modified CFE and explore the dynamic changes of dopamine and 5-hydroxytryptamine in vivo. It is anticipated that a successful outcome of this study will greatly promote the advancement of in vivo electroanalytical chemistry and lay the foundation for understanding the chemical essence of brain function and the development of neurological diseases.