蓝氏贾第虫exosomes的鉴定和功能研究

贾第虫病人兽共患，危害严重。虽然有关蓝氏贾第虫与宿主互作的机制研究已长期开展，但主要聚焦在蓝氏贾第虫分泌蛋白与宿主互作方面。最近研究发现多种寄生虫exosomes能够向宿主细胞传递miRNAs，参与宿主免疫反应。前期研究发现蓝氏贾第虫能够释放exosomes，且在贾第虫病人十二指肠及粪便中均存在虫源性miRNAs。我们推测蓝氏贾第虫exosomes可能参与虫体与宿主互作。因此，本研究拟从exosomes的功能入手，分离并鉴定蓝氏贾第虫exosomes的主要组份，研究虫源性exosomal信息在宿主细胞中的传递及对宿主细胞免疫因子及mRNA表达谱的影响，解析exosomal miRNAs对宿主靶向基因的调控作用。预期结果将揭示蓝氏贾第虫exosomes在虫体与宿主互作中的分子调控机制，为深入探讨蓝氏贾第虫的分子致病机理提供参考。

Giardiasis is a zoonotic disease that poses a significant threat to public health. Although the research on the mechanism of G.lamblia-host interactions has been carried out for a long time, it mainly focuses on the interactions between G.lamblia excretory secretory proteins and host. Recent studies have found that exosomes secreted by many parasites can transfer miRNAs to host cells and participate in host immune response. Recent studies have shown that G.lamblia can release exosomes and miRNAs were found in duodenum and feces of patients infected with G.lamblia. Our hypothesis is that exosomes secreted by G.lamblia play a role in parasite-host interaction. In this research, we aim to identify the main component of the exosomes, study the transfer of exosomal information to host, evaluate the impact of exosomes on host cells, and further investigate the function of exosomal miRNAs on their targets. The results are expected to provide new insights for understanding the molecular mechanism of G.lamblia-host interaction.