胎鳉科鱼类胎生相关特征——排卵抑制的分子基础研究

卵生到胎生的转变是脊椎动物演化历程中最为重大的事件之一，此过程分子机制的研究能够解答相应的生理生态、表型、行为等方面的生物学问题。胎鳉科作为鱼类胎生演化研究的代表类群，其胎生的先决条件是排卵抑制（体内妊娠维持实现的重要前提），然而关于这一重要特征的分子基础研究仍待开展。基于申请人已有的研究基础，本项目以胎鳉科鱼类为研究对象，通过与卵生类群的比较基因组学分析并结合西部食蚊鱼（胎鳉科代表物种）不同发育时期卵巢的ATAC-seq和RNA-seq数据以及RT-PCR来筛选胎鳉科特有的基因和保守元件，将获得的结果以西部食蚊鱼为模型进行相应的In situ hybridization和Dual-luciferase assay实验，以对胎鳉科鱼类排卵抑制这一重要胎生特征的分子基础进行初步探究。本项目的实施与完成有望为鱼类胎生演化分子机制的全面解析奠定基础。

The transition from oviparous birth to viviparous birth is one of the most significant events in the evolution of vertebrates. The study of the molecular mechanism of this process can answer the corresponding biological questions in physiological ecology, phenotype, and behavior. Poeciliidae is a representative family of fish that is used widely in viviparous evolution research, their prerequisite for viviparous birth is ovulation inhibition (an important prerequisite for achieving pregnancy maintenance in vivo), but the research on the molecular basis of this characteristic has not yet been carried out. Based on the applicant’s existing research foundation, this project uses poeciliid fish as a model, and screens the unique genes and conserved regions in poeciliid fish through comparative genomic analysis with oviparous groups, the results will be filtered by combining ATAC-seq and RNA-seq data from ovaries of western mosquitofish (representative species of Poeciliidae) at different stages and RT-PCR, and the results conducted In situ hybridization and Dual-luciferase assay experiments in western mosquitofish to initial research the molecular basis of ovulation inhibition in Poeciliidae. It will lay a foundation for the comprehensive analysis of the molecular mechanism of fish viviparous evolution.