赤霉素调控毛白杨剥皮再生过程中形成层及次生维管组织模式形成的机理研究

木材来源于树木次生维管组织发育产生的次生木质部，由形成层细胞分裂、分化形成。生长素和细胞分裂素可能作为位置信息参与调控次生维管组织发育，然而，赤霉素在其中的调控作用所知甚少。本项研究拟以毛白杨为材料，利用剥皮再生和管状分子分化诱导实验体系，研究赤霉素对形成层发生及次生维管组织模式形成的影响，进一步利用遗传学、生物化学和分子生物学方法研究赤霉素与生长素和细胞分裂素在次生维管组织发育过程中的相互作用关系，揭示赤霉素调控毛白杨剥皮再生过程中形成层发生及次生维管组织模式形成的分子机理，为木材形成的理论研究以及生产实践提供实验依据。

Wood, production of the secondary vascular tissue development of trees, derives from the cambial cell division and differentiation. Auxin and cytokinin involve in regulating secondary vascular tissue development maybe as position signals, while a little is known about the roles of gibberellin during the process. This study intends to investigate the roles of gibberellin on cambium and secondary vascular tissue development in Populus tomentosa by using in vitro secondary vascular tissue regeneration after bark girdling and the ectopic tracheary element induction system, combing with genetics, biochemistry and molecular biology techniques. The results of this study will provide new information for theoretical research and practical utilization of wood formation.