番茄花序形态建成的遗传基础与调控网络

番茄花序形态决定果实数量和果实大小，是影响番茄产量的一个重要性状。番茄花序发育模式非常独特也很复杂，目前对其形态建成分子机制的认识非常有限，限制了育种中对该性状的选择和利用。申请人前期获得了一个番茄无限花序株系ST024，通过遗传分析确定了该株系表型由4个位点共同决定，并发现MBP21及AP2-like是参与调控其花序形态建成的其中2个基因。在此基础上，我们将进一步克隆另外2个参与ST024花序调控的基因，揭示这些基因之间的遗传效应和相互作用关系，解析ST024表型产生的遗传基础。通过转录组、染色质免疫共沉淀及蛋白互作筛选系统等分析MBP21等转录因子调控花序形态的直接作用靶点及相互作用蛋白，解析番茄花序形成的分子调控机制和网络。该研究将揭示番茄花序形成的分子机制，丰富和深化自然界花序结构多样性的理论基础，同时为番茄品种选育提供支撑。

The shape of inflorescence in tomato determines fruit number and size, and affects its yield. As a model plant of sympodial species, tomato inflorescence is cymes, which is complicated and different from Arabidopsis and rice. Many factors affect the inflorescence development in tomato. However, limited knowledge about its development blocks the selection and application of this trait in tomato breeding. We found a recombinant inbred line ST024, which has indeterminate inflorescence, and four loci controlling the morphology of ST024 by genetic analysis. We have been cloned two genes, MBP21 and AP2-like，and verified their function in inflorescence development. In this work, we will further clone the other two genes， and determine their function. We will explore the genetic effects of these genes and their relationship in regulating inflorescence meristem identification. We will also study their protein interaction， and identify their direct targets to reveal the molecular mechanism of these genes during the inflorescence development in tomato. Our results will reveal the molecular mechanism of inflorescence development in tomat and provide the genetic base for tomato breeding.