唾液蛋白exportin-6调控灰飞虱取食水稻的分子机制

刺吸式昆虫的唾液蛋白是调控植物防御反应的主要效应因子。灰飞虱是水稻上的重要害虫，通过取食和传播病毒导致水稻严重减产，而对灰飞虱唾液蛋白的功能和作用机制仍缺乏足够的认识。本项目拟深入研究一个新的唾液蛋白exportin-6对灰飞虱取食水稻的作用及机理。研究内容包括三部分：通过唾液、唾液腺和血淋巴的免疫分析及各组织的表达分析，明确exportin-6的表达和分泌是否具有组织特异性；结合昆虫RNA干扰、水稻双链小RNA过表达和基因过表达技术研究exportin-6对灰飞虱取食和生存的影响；比较过表达exportin-6转基因水稻和野生型水稻的基因表达差异，明确exportin-6通过调控水稻哪些抗性通路而影响灰飞虱对水稻的适应性。研究成果不仅揭示唾液效应因子介导昆植互作领域新的分子机制，阐明exportin家族核转运以外的新功能，而且为未来开发新的飞虱防控技术提供理论依据。

Saliva proteins are main effectors of piercing-sucking insects in regulating plant defense reaction. The small brown planthopper is one of predominant pest insects in rice and causes serious reduction in rice production due to feeding and transmission of rice viruses. The roles and functional mechanisms of saliva proteins from small brown planthoppers are far from well known. In the present program, we will work on a new saliva protein of small brown planthoppers, exportin-6, to explore its role in affecting insect feeding on rice and the underlying mechanisms. The contents of this study include clarifying the expression of exportin-6 in different organs and the secretory characteristics using immunoblot on saliva, salivary glands and hemolymph; exploring the effects of exportin-6 on feeding and survival of planthoppers by insect RNAi and the transgenic rice expressing double-stranded RNAs or proteins of exportin-6; and finding the defense pathways regulated by exportin-6 through the transcriptomic analysis on gene expression variations between the exportin-6 transgenic and wild rice. The research outcome will not only elucidate new mechanisms of effector-mediated interaction between insects and host plants and new functions of exportin family apart from the nucleus transportation, but also provide a theorical basis for developing new techniques for control of planthoppers.