低聚有机磷酸酯BDP在生态修复型挺水植物根际和体内的迁移转化机制

双酚A双（二苯基磷酸酯）（BDP）是国内需求高的一种低聚有机磷酸酯阻燃剂，其在太湖沉积物中的赋存与单体有机磷酸酯的总量相当，鉴于其代谢产物双酚A的危害，BDP在水生植物根际的降解、以及被植物吸收并在体内代谢又经腐解释放的行为亟待解析。本项目拟针对生态修复常用的挺水植物，研究BDP在其根际和体内的迁移转化机制。首先，研究根际细菌和根系分泌物的响应，用根系分泌物培养优势菌群并筛选降解菌，阐明根系分泌物介导下根际优势菌群降解BDP的调控机制；其次，研究植物吸收BDP的动力学及BDP在皮层、维管束和亚细胞的分布，结合蛋白组学分析揭示植物吸收和运输BDP的途径及转运蛋白；最后，采用转录组学技术分析植物体内BDP代谢酶，并研究微生物对腐解释放的作用，探明植物体内BDP的酶代谢和腐解释放过程。本项目有助于预测真实环境中BDP的归趋，支持BDP生态风险评估模型的建立，推动BDP污染治理的植物修复技术应用。

Bisphenol A bis(diphenyl phosphate) (BDP) is a popular oligomeric organophosphorus flame retardant with high demand in China. It has been detected in the sediment of Taihu lake with a concentration comparable to the sum of traditional monomeric organophosphorus flame retardants. Due to its harmful metabolite bisphenol A, it should be paid great attention to the biodegradation of BDP in the rhizosphere of aquatic plants, and also to the uptake of BDP by aquatic plants followed by the in vivo transformation and the decomposing release. The main objective of this project is to explore the rhizospheric and in vivo translocation and transformation mechanisms of BDP for the emergent macrophytes commonly used in ecological restoration. Firstly, we will investigate the characteristics of rhizosphere bacteria and root exudates in response to BDP, cultivate the dominant bacteria with root exudates, and screen the BDP degrading bacteria to clarify how root exudates regulate the dominant rhizosphere bacteria to degrade BDP. Secondly, we will study the uptake kinetics of BDP and the distribution of BDP in the cortex, vascular bundles and subcelluar fraction. And then combined with the proteomic analysis, we will reveal the uptake and transportation pathway of BDP by plants and the associated transport proteins. Finally, transcriptomics will be used to analyze the enzymes able to metabolize BDP in plants, and the role of microorganisms in plant decomposition will be also studied, aiming to elucidate the enzyme-catalyzed metabolism of BDP inside plants and the release of BDP and its metabolites via decomposing process. This project will help predict the fate of BDP in the real environment, support the establishment of ecological risk assessment model for BDP, and promote the application of phytoremediation technology for BDP pollution control.