用于引导骨再生的非共价键增强甲壳素/生物陶瓷材料研究

引导骨再生（GBR）是治疗骨缺损的有效手段之一，临床尚缺乏兼具可降解，高强度及刚度（杨氏模量）（保证缺损部位稳定）的GBR材料。本项目拟以生物可降解的甲壳素及羟基磷灰石（HA）为基元，针对甲壳素与生物陶瓷界面作用力较弱及氢键连接的甲壳素分子链具有少量活动能力两项对实现其复合材料理想力学性能的不利因素，引入非共价键受体，在保留甲壳素及HA生物材料属性的基础上，在甲壳素与HA界面及甲壳素三维网络中形成多种非共价键作用,同时增强甲壳素/HA界面相互作用及甲壳素分子链间作用力，结合仿贝壳多层级结构设计及热压诱导自组装，集成出在拉伸和弯曲下都具有高强度及刚度的甲壳素/HA材料。以骨髓间充质干细胞及颅骨缺损模型探讨材料结构及性能对骨缺损修复作用的影响及相关机制。本项目将为通过物理方法增强甲壳素本征网络及其杂化材料界面作用提供新方法，有望为GBR材料的开发和设计提供新思路，具有重要的学术意义和应用前景。

Guiding bone regeneration (GBR) is an effective method for curing the bone defects. There is still no GBR materials in clinical practice, which could meet all the following demands including in vivo biodegradation, high strength and rigidity (high Young’s modulus) (ensuring the structure stability of the bone defects area). In this project, we plan to use the biodegradable chitin and hydroxylapatite (HA) for constructing the high strength and stiff composite films. Considering the negative effects of the two factors (1. the weak chitin/HA interaction, and 2. the small mobility of the chitin chains resulting from the hydrogen bonding interaction mode among the chitin chains) on the mechanical properties of the composite materials, the noncovalent receptor will be introduced to enhance the chitin/HA interface interaction and the chitin network through forming the noncovalent bonds at the chitin/HA interface and within the chitin networks. Moreover, the biomaterial attributes of chitin and HA will be maintained in this process because that no new covalent bond will form. The further nacre structure design and hot press induced self-assembly method will be applied to produce the chitin/HA composite materials with the hierarchical structure, which exhibits the high strength and rigidity at both the tensile and bending state. The mesenchymal stem cell and the calvarial defect model will be used to evaluate the affections and action mechanism of the chitin/HA composite materials structure and properties on the bone defects regeneration. It is expected that the project will open a new avenue for enhancing the chitin network and chitin based hybrid material interface interaction and providing new method and strategy for GBR materials preparation, showing important scientific significance and practical value.