抗菌性稳定化分子筛负载Fe@Cu的构型及其去除抗生素抗性基因的机理

抗生素抗性基因（ARGs）是重要的新兴污染物，如何高效去除ARGs是控制抗生素耐药性传播亟待解决的问题。申请者前期探索发现茶叶提取物稳定化纳米零价铁（T-nZVI）可提升纳米零价铁（nZVI）的反应活性，获得更高的ARGs去除效能，但反应机制尚不明确。在此基础上，本项目以高效去除ARGs为目标，针对nZVI易钝化、中/碱性条件下Fe0腐蚀速率低的问题，提出构建稳定化分子筛负载型Fe@Cu复合材料（T-Fe@Cu-MCM-41）去除ARGs的研究思路。重点探究稳定化技术对nZVI粒径、晶型、表面电荷、抗菌性和反应活性等的影响，通过多因素正交试验分析ARGs去除的变化规律，解析T-Fe@Cu-MCM-41去除ARGs的构效关系；明确电子供体Fe2+产率与ARGs去除率的关系；明晰茶多酚等生物活性物质的反应增效方式；阐明ARGs去除的作用机制，为nZVI在环境中ARGs去除中的应用奠定理论基础。

Antibiotic resistance genes (ARGs) are important new contaminants. How to remove ARGs efficiently is an urgent problem in the control of the spread of antibiotic resistance.The applicant's previous exploration found that the stabilization of nano-zero-valent iron (T-nZVI) by tea extracts can improve the reactivity of nano-zero-valent iron (nZVI) and shows higher ARGs removal performance, but the synergistic mechanism remains unclear. On this basis, this project aims at the efficient removal of ARGs. Aiming at the problems of easy passivation of nZVI and low corrosion rate of Fe0 under medium/alkaline conditions, a research idea of constructing a green stabilized molecular sieve loaded Fe@Cu (T-Fe@Cu-MCM-41) to remove ARGs was proposed. The influence of green stabilization on particle size, crystal shape, surface charge, antibacterial activity and reactivity of nZVI will be explored. The change rule of ARGs removal under multiple factors will be analyzed by orthogonal test, and the structurally-activity relationship of T-Fe@Cu-MCM-41 will be analyzed. The relationship between Fe2+ which act as the electron donor and ARGs removal efficiency will be determined. The synergistic ways of reaction with tea polyphenols and other bioactive substances will be clarified; The mechanism of removing ARGs will be elucidated.The research conclusions of this project will lay a theoretical foundation for the application of nZVI in the removal of ARGs.