转录因子TFIID通过miR-134影响食管鳞癌上皮间质转化的机制研究

河南与山西、河北三省交界的太行山区是世界上食管鳞癌（ESCC）发病率和死亡率最高地区。浸润转移是其主要死亡原因。团队在1项国自然和5个省级项目支持下，已完成食管癌高发区1000余例ESCC及癌前组织样本收集和临床随访数据库建设。通过microRNA表达谱、生信分析等发现转录因子TFIID、miR-134在ESCC中功能及临床意义显著。预实验发现TFIID、miR-134均能影响PLXNA1、c-Myc和EMT表型，且TFIID、miR-134及PLXNA1存在直接互作。因此，我们提出：ESCC中TFIID可能通过miR-134影响EMT表型。围绕此假说，拟开展芯片检测、分子直接互作、功能回复及大样本验证，并整合已完成的高通量筛选等数据库，探索TFIID与miR-134-PLXNA1-c-Myc及EMT、浸润转移的关系，为建立ESCC高危人群筛查、精准诊疗分子标志物提供支持。

The Taihang Mountains, located at the junction of Henan, Shanxi and Hebei provinces, are the highest incidence rate and mortality rate of esophageal squamous cell carcinoma (ESCC) in the world. Infiltration and metastasis are the main cause of death. With the support of one NSFC and five provincial projects, the team has completed the construction of more than 1000 ESCC and precancerous tissue samples and clinical follow-up database in high incidence areas of esophageal cancer. It was found that TFIID and miR-134 had significant function and clinical significance in ESCC by microRNA expression profile and bio information analysis. It was found that TFIID and miR-134 could affect PLXNA1, c-myc and EMT phenotypes, and TFIID, miR-134 and PLXNA1 had direct interaction. Therefore, we suggest that TFIID may target miR-134, and then affect EMT of ESCC. Based on this hypothesis, chip detection, direct interaction, functional recovery and large sample verification are proposed. And the relationship between TFIID and miR-134-PLXNA1-c-myc, EMT, infiltration and metastasis was explored. To provide support for the establishment of ESCC high-risk population screening and accurate diagnosis and treatment.