DUF4228基因家族在拟南芥非生物胁迫应答中的功能解析

干旱、低温和盐等非生物胁迫是影响植物生长和产量的重要因素。研究表明，众多未知功能DUF基因参与植物对非生物胁迫的响应，然而分子机理仍不清楚。课题组前期的研究表明，拟南芥DUF4228家族基因与植物抗逆相关，其中的一个基因负调控植物抗逆能力。本项目拟对植物DUF4228家族成员进行全基因组鉴定，利用FOX-hunting技术获得拟南芥DUF4228过表达库。通过对转基因植物干旱、冷和盐胁迫条件下的表型筛选，结合基因非生物胁迫下的表达谱、启动子响应元件和蛋白互作分析，挑选拟南芥DUF4228家族中抗逆相关的重要基因。利用过表达和突变体验证其生物学功能，通过转录组、蛋白组关联分析，酵母双杂等手段确定其信号通路。通过上述研究，明确DUF4228基因家族在植物抵抗非生物胁迫中的生物学功能和分子机制。本项目的研究结果为进一步丰富植物非生物胁迫响应信号通路，增加了解植物抗逆的分子机制提供理论基础。

Abiotic stress, such as drought, cold and salinity, is one of major factors that affect plant growth and yield. A large number of researches have indicated that DUF genes are involved in plant response to abiotic stress, but the underlying mechanisms are still elusive..In previous experiments of this project, we found that DUF4228 genes of Arabidopsis are involved in plant resistance to abiotic stress, and one of them (AT3G03280) negatively regulates plant stress resistance. In this project, base on genome-wide identification of DUF4228 in plant, the FOX-hunting technology was use to obtain the ATDUF4228 overexpression library. The important stress-related genes of ATDUF4228 were selected, relying on phenotypic of transgenic plantunder drought, cold and salt stress, gene expression profiles, promoter response elements and protein interaction networks analysis. Overexpression and mutants lines were used to verify their biological functions, and their signaling pathways were determined by transcriptome, proteome, and yeast double hybrids..This project will significantly help us to learn about the mechanism of abiotic stress resistance in plant and the biological function of DUF4228. The results of this project will be helpful for us to further understand abiotic stress signaling pathway and provide theoretical basis to understand molecular mechanisms of plant stress resistance.