MYB转录因子BnWERs调控甘蓝型油菜根毛发育的分子机制研究

根毛(Root hairs)对根系吸收土壤水分和矿质养分起关键作用，因此对油菜养分吸收、抗逆和产量均很重要。前期我们在油菜基因组中筛选/克隆了4个R2R3-MYB转录因子基因(简称BnWERs)，通过功能互补、超量表达等实验证明了候选BnWERs对根毛发育具有调控作用。本项目拟在此基础上：(1)利用基因沉默、超量表达、功能互补等实验明确候选BnWERs对油菜根毛发育的调控作用及其与养分吸收、抗逆、产量的关系；(2)结合ChIP-Seq、共表达分析和多组学实验鉴定候选BnWERs调控的靶基因和靶位点，阐明其分子机制；(3)通过Y2H、BiFC和Co-IP-MS实验探究候选BnWERs蛋白的互作蛋白及其互作机制；(4)解析候选基因及其靶基因在油菜重测序材料中的遗传变异规律及其与养分吸收、抗逆、产量的关系。本项目将促进油菜根毛发育的基础研究，为高产分子育种提供理论依据和基因资源。

Root hairs play a key role in plant absorbing of water and mineral nutrients distributed in soil, thereby is important for nutrient absorption, stress resistance, and yield of Brassica napus. Four R2R3-MYB transcription factor genes (BnWERs) were identified and cloned from B. napus genome in our previous study. Subsequently, the regulating roles of these four candidate BnWERs in root hairs development were confirmed by functional complementation assay, over-expression assay, etc. Based on those results, the present project will focus on the following four issues: (1) confirming the roles of candidate BnWERs involved in regulating B. napus root hairs development, and their impact on B. napus nutrient absorption efficiency, stress resistence, and yield, by gene silencing (gene editing), over-expression, and functional complementation experiments; (2) identifying the downstream target genes and binding sites of candidate BnWERs proteins in B. napus genome, and their regulating mechanisms involved in root hairs development by ChIP-Seq, Co-expression analysis, RNA-Seq, and Multi-Omics analysis; (3) identifying the interaction proteins and the protein interaction mechanisms of candidate BnWERs proteins by yeast two hybrid assay (Y2H), Bimolecular fluorescence complementation (BiFC), and Co-immunoprecipitation and mass spectrometry assay (Co-IP-MS); (4) exploring the genomic variation profiles of candidate BnWERs and their target genes in 600 re-sequencing B. napus gerplasms, and the correlation between the genomic variation profiles of candidates and the nutrient uptake efficiency, stress resistence, and yield of B. napus, etc. Our results will promote the fundamental research of B. napus root hairs development as well as provide theoretical foundation and gene resources for Molecular Breeding to improve B. napus yield.