质膜和细胞骨架在葡萄抗间座壳菌Diaporthe eres免疫信号转导中的作用机制

葡萄蔓枯病（Diaporthe dieback）是全球范围内重要的葡萄枝干病害（Grapevine Trunk Diseases,GTDs）之一。目前，27 种间座壳属（Diaporthe spp.）真菌被发现同蔓枯病相关。其中，Diaporthe eres在我国分布范围最广，影响最大。本人前期研究表明，葡萄枝干病害（GTDs）相关真菌的胞外分泌物可诱导葡萄内源免疫反应，包括细胞骨架（包括微管和肌动蛋白）重组、化学信号转导、抗性基因表达等，同时证实，质膜和细胞骨架也参与调控葡萄早期免疫信号转导的作用。在此基础上，本研究以不同细胞骨架蛋白被GFP标记的葡萄细胞悬浮系和葡萄组培苗为试材，结合转盘式共聚焦显微镜技术，探究质膜和细胞骨架对葡萄蔓枯病菌D.eres胞外分泌物的感知功能以及相关免疫信号传递中的作用机制，为葡萄抗病育种提供理论依据和研究基础。

Diaporthe dieback，one of the important Grapevine Trunk Diseases (GTDs), has been reported to be caused by 27 fungal species of Diaporthe spp. Among them, Diaporthe eres has the widest distribution and the greatest influence in China. Previous studies have shown that the extracellular secretions of fungi related to GTDs can induce endogenous immune responses in grapevine, including cytoskeleton (including microtubules and actin filaments) reorganization, chemical signal transduction, and resistance gene expression, etc. Meanwhile, it was confirmed that the plasma membrane and cytoskeleton are also involved in the regulation of early immune signal transduction in the grapevine. Therefore, the current study uses grapevine suspension cells with different cytoskeletal proteins labelled with GFP and grapevine seedlings as the test materials. The cytoskeleton responses induced by the Diaporthe eres extracts will be observed by the spinning disc confocal microscope technology and be quantified by ImageJ. Further, D. eres secretions induced grapevine immunity responses and signals transduction will be measured. In summary, this study will provide theoretical guidance for the prevention and cure of the Diaporthe dieback.