黔产药用蕨类芒萁属植物内生真菌多样性及生物活性研究

特殊环境真菌是药物先导分子和候选药物的重要源泉，因其长期适应特殊生境进化出异于普通环境真菌的独特次级代谢网络途径，从而能合成出结构新颖、生物活性显著和作用机制独特的次级代谢产物。申请人团队前期预实验研究发现，贵州药用蕨类芒萁属植物内生真菌受溶洞、裂隙和沟槽等特殊喀斯特微生境的影响，其真菌物种丰富多样，部分菌株具有抗癌和抗菌活性，极有可能成为创新药物发现的重要微生物资源。然而目前缺乏针对黔产芒萁属蕨类植物内生真菌的系统研究，其物种构成及多样性分布格局未明确，导致这类真菌资源没有得到开发利用。因此，本项目拟开展黔产药用蕨类芒萁属植物内生真菌多样性分布格局及其代表性菌株的生物活性研究。本研究结果将完善黔产芒萁属植物内生真菌研究体系，发掘新的真菌物种和活性菌株，丰富菌种资源库，为发现新结构和新作用机制的活性次级代谢产物提供资源基础。

The fungi in special environments have been shown as important resource for discovering lead molecules and drug candidates. Due to their long-term adaptation to special habitats, they have evolved a unique secondary metabolic network pathway which is different from those found in general environments, thus they can synthesize novel secondary metabolites with new structures, significant biological activities and unique mechanism of action. The studies carried by the applicant’s research team have found that Guizhou’s medicinal Dicranopteris ferns fungi are affected by unique Karst microhabitats such as caves, fissures and grooves. Those fungi associated with medicinal Dicranopteris ferns are rich and diverse, and some of the strains exhibit anticancer and antibacterial activities which have the great potential to become important microbial resources for innovative drug discovery. However the research on the Dicranopteris ferns fungi from Guizhou is poorly studied, and its species composition, diversity and distribution pattern remains less documented and it has heavily hold the development and utilization of the fungal resources in terms of applications. Therefore, the aims of this project are: 1) to study the endophytic diversity, community structure and distribution pattern of three medicinal Dicranopteris ferns (Dicranopteris ampla, D. linearis and D. pedata) in the typical Karst microhabitat of Guizhou; 2) to carry out screening of anticancer and antibacterial activities from our further obtained medicinal fern strains such as the new and dominant species, and to provide basic resources for the big health industries such as biopharmaceuticals. This study has important theoretical significance and application value for the taxonomic study of the medicinal ferns fungi and it will contribute the knowledge of the fungal resources which have huge potential in industrial application.