黄芪、莪术配伍对肿瘤相关成纤维细胞介导的肝癌早期转移的调控机制

肿瘤细胞的转移已经成为肿瘤难以治愈的重大问题之一，寻找并阻断肿瘤转移的关键性因素，具有重要的潜在研究价值。既往认为肿瘤的转移属于晚期事件，最近的研究显示：在肿瘤发生的早期阶段，肿瘤相关成纤维细胞就与癌细胞“沆瀣一气”，从原发部位“劫持”癌细胞进行血行扩散。血小板源性生长因子是促进肿瘤相关成纤维细胞介导的早期肿瘤转移的关键因子。针对肝癌走窜难遏之转移特点和气虚血瘀之病机关键，补气活血药黄芪、莪术相使配伍，复本祛邪，补通兼施，共奏抑瘤削结、固摄癌瘤之功。结合本课题组前期研究结果：黄芪、莪术配伍能够诱导人肝癌细胞HepG2凋亡，抑制肝癌血管生成，联合化疗药减少结肠癌的转移。因此本课题组建立肿瘤相关成纤维细胞与肝癌HuH7细胞体外共培养系统和斑马鱼肝癌模型，观察黄芪、莪术配伍对肿瘤相关成纤维细胞介导的肝癌早期阶段的转移情况及分子机制，旨在拓展补气活血药物的临床价值，同时丰富中药配伍理论。

Metastasis of tumor cells has become one of the major problems that are difficult to cure tumors. It is of great potential research value to find and block the key factors of tumor metastasis. Metastasis has been considered a late event. Recent studies have shown that: In the early stages of tumor development, cancer associated fibroblasts (CAFs) associate with the cancer cells, “hijacking” the cancer cells from the primary site to proliferate. Platelet-derived growth factor is a key factor in promoting CAFs -mediated early tumor metastasis. In view of the characteristics of hepatocellular carcinoma metastasis and the pathogenesis of qi deficiency and blood stasis, radix astragali and curcuma were combined to strengthen body resistance and eliminate evil, and to inhibit cancer. Combined with the previous research results of our research group：The combination of radix astragali and curcuma can induce the apoptosis of human hepatocellular carcinoma cells HepG2, inhibit the angiogenesis of hepatocellular carcinoma, and reduce the metastasis of colon cancer. Therefore, our research group established CAFs co-culture system with HuH7 cells in vitro and hepatocellular carcinoma metastasis model in zebrafish. We observed the effect of radix astragali and curcuma on the early stage of CAFs-mediated hepatocellular carcinoma metastasis and its molecular mechanism. The aim of this study was to expand the clinical value of the drugs for invigorating qi and breaking blood and enrich the compatibility theory of traditional Chinese medicine.