水稻CTB18基因调控孕穗期耐冷的遗传和分子机制解析

水稻孕穗期对低温异常敏感，发生在该时期的障碍型冷害严重威胁我国水稻生产安全。深入了解水稻孕穗期响应低温的调控机制，进而在分子设计水平上培育耐冷水稻品种是从根本上解决障碍型冷害问题的重要途径，但目前对调控水稻孕穗期耐冷机制的了解还十分有限。本项目拟在前期通过诱变筛选结合遗传分析鉴定到的水稻孕穗期耐冷基因COLD TOLERANCE AT BOOTING STAGE 18（CTB18）的基础上，综合运用分子遗传学、细胞生物学、生物化学、脂质组学等多种研究手段，系统解析CTB18编码的酰基载体蛋白调控水稻孕穗期耐冷的分子机制和调控网络，挖掘优异自然变异位点，为运用基因编辑、分子育种等技术快速选育孕穗期耐冷水稻品种提供理论基础和遗传资源。

Rice is hypersensitive to low temperature at booting stage and sterile-type cold injury occurred at this stage threatens national rice production security severely. An important approach to overcome sterile-type cold injury is to breed cold-tolerant rice varieties using molecular design tools based on our knowledge of mechanisms underlying cold response at booting stage, which still remain elusive. We previously identified a rice cold tolerance gene at booting stage, named CTB18 (COLD TOLERANCE AT BOOTING STAGE 18), by genetic analyses of a mutant obtained by EMS mutagenesis. In this project, we will decipher the mechanisms and regulatory network of the acyl carrier protein encoded by CTB18 in cold response at booting stage of rice using methods of molecular genetics, cellular biology, biochemistry and lipidome synthetically. Together with the mining natural variations for potential application, the results will provide valuable insights, new strategies and genetic resources for breeding cold-tolerant rice varieties by genome editing and molecular design methods.