羽毛针禾对其根鞘核心微生物组的遗传塑造作用及调控机制

羽毛针禾是优良的固沙先锋植物，具有抗旱、耐风蚀等适应流动沙丘的特征和丰富的抗逆基因资源。如何科学利用羽毛针禾防沙治沙，已成关注点。然而，羽毛针禾与其根鞘微生物组的互作机制，仍然匮乏。.本组前期关联了羽毛针禾基因组变异与根鞘微生物组的多样性（mGWAS），发现宿主遗传影响力已深入微生物组的进化历程，提出“宿主遗传变异塑造了核心微生物组”的科学假设。然而，其中深层的遗传效应及调控机制，几为空白。.本项目预以前期mGWAS的关联效应为基础，深入解析羽毛针禾塑造其根鞘核心微生物组的加性、显性、隐性、上位等遗传效应；进而，在羽毛针禾基因组上，定位微生物组数量性状基因座（mQTL），鉴定紧密连锁基因，挖掘宿主调控微生物组的分子通路；最后，阐明可遗传的核心微生物组，揭示羽毛针禾对其根鞘核心微生物组的遗传塑造作用及调控机制，为荒漠治理，为以增强微生物（群）性状为方向的农业可持续发展，提供新思路和新靶点。

Stipagrostis pennata is an excellent sand-fixing plant. It has the drought and wind-erosion resistance which helps it to adapt to the mobile dunes, and it is rich in the gene resources of stress resistance. How to use Stipagrostis pennata scientifically has become the focus of sand-fixing work. However, the interaction mechanism between Stipagrostis pennata and its rhizosheath microbiome remains to be elucidated...In the preliminary work of our group, we analyzed the association between the genomic variation of Stipagrostis pennata and the diversity of its rhizosheath microbiome (mGWAS). We concluded that the genetic influence of the host has penetrated into the evolution of the microbiome, so we proposed the hypothesis that "host genetic variation shapes the key microbiome". However, detail genetic effects and regulatory mechanisms of the hypothesis are uncharted...Based on the association results from the previous mGWAS work, we will further analyze the genetic effects, such as additive, dominant, recessive, and epistatic effects, of Stipagrostis pennata on the key microbiome in its rhizosheath. We will then locate the microbiome quantitative trait locus (mQTL) in Stipagrostis pennata's genome and identify genes closely linked to the mQTL. We will explore the molecular pathways of the host to regulate the microbiome. Finally, we will identify the key heritable microbiome and reveal the genetic effect and regulation mechanism of Stipagrostis pennata on shaping the key microbiome in its rhizosheath. We hope to provide novel targets and ideas for the desert control and the sustainable development of agriculture focused on enhancing microbial (group) traits.