CYP4G188介导20E调控桔小实蝇围蛹发育的分子机理

本项目以果蔬危险性害虫桔小实蝇为研究对象，紧密围绕CYP4G188介导20E调控桔小实蝇围蛹发育的分子机理这一关键科学问题，综合运用RNAi、S2细胞真核表达、CO差光谱、超景深三维显微系统、HPLC等技术和手段，在前期围蛹发育形态特征鉴定及CYP4G188时空表达模式解析的基础上，系统分析桔小实蝇显头蛹-隐成虫转变期的形态学变化特征，阐明20E对桔小实蝇围蛹发育进程及CYP4G188表达的调控模式，进而明确CYP4G188参与桔小实蝇蛹-成虫蜕变期20E的代谢过程，最终揭示CYP4G188介导20E调控桔小实蝇围蛹发育的分子机理。研究结果不仅有助于解析昆虫变态发育的调控机理，还有利于寻获新的杀虫靶标，为实蝇类害虫防控提供新的思路，因此具有重要的理论意义和应用前景。

The research focuses on the molecular action of CYP4G188-mediated 20E in intrapuparial development of the oriental fruit fly, Bactrocera dorsalis, one of the most economically important fruit fly pests devastating fruit and vegetable industry. On the basis of previous study about morphological identification of intrapuparial development and spatiotemporal expression analysis of CYP4G188, a series of molecular techniques including RNAi, eukaryotic expression in Drosophila S2 cells, carbon monoxide differential spectrum, ultra-depth three-dimensional microscope and high performance liquid chromatography are utilized comprehensively to study the morphological changes during the transition between the phanerocephalic pupae and pharate adults; to examine the effects of exogenous 20E on intrapuparial development and its regulation pattern on CYP4G188; to analyze metabolism process of CYP4G188 on 20E during intrapuparial development; finally to clarify the molecular mechanism of CYP4G188-mediated 20E in intrapuparial development of the fruit fly. The results will enrich the knowledge about regulation mechanism of insect metamorphic development, promote to seek novel insecticide targets and provide new control strategy on the fruit flies. Therefore, it has important theoretical significance and application prospects.