基于Caco-2细胞模型研究木瓜中农药多残留联合暴露的吸收转运、毒性效应及健康风险

中药材农药残留经膳食摄入进入人体后，会以低剂量、多残留暴露特征产生联合毒性效应。肠道是外源化合物吸收的主要场所，天然产物以及环境污染物经肠吸收转运过程中的相互作用是影响其联合毒性效应的重要因素。农药残留联合暴露经肠吸收转运过程中会发生怎样的相互作用？目前未见报道。明确农药残留在吸收转运过程中的联合毒性效应是当前准确评价中药材中农药多残留联合暴露风险亟待解决的科学问题。本项目以药食同源木瓜中高频检出的农残为对象，采用人源结肠癌Caco-2细胞模型，系统研究农药多残留在吸收转运过程中的相互作用规律及其影响Caco-2细胞屏障功能的联合作用模式，建立不同类型农药残留联合毒性阈值的预测模型，定量评估木瓜中农药多残留对人体健康的联合暴露风险。研究结果对于科学评价木瓜中农药多残留的健康风险、加强中药材农药残留污染的源头控制和风险管理具有重要的理论指导意义与实际应用价值。

Pesticide residues in Chinese herbal medicines will be low-dose, multi-residual exposure characteristics of joint toxicity after dietary intake into the human body. The intestinal tract is the main site of absorption of foreign compounds, and the interaction of natural products and environmental organic pollutants is an important factor affecting their toxic effects. How does co-exposure to pesticide residues interact with intestinal transport? It has not been reported. It is a scientific problem to evaluate the risk of joint exposure of pesticide residues in Chinese herbal medicines in order to clarify the interaction and toxicity of joint exposure during absorption and transport. Caco-2 cell model is used to study the interaction of different pesticide residues combinations and their joint toxicity to intestinal cell barrier function during absorption and transport, and a threshold prediction model for pesticide residues joint toxicity was established based on Caco-2 cell proliferation to assess the combined exposure risk of pesticide residues in Chaenomeles speciosa. It’s great theoretical significance and practical value for evaluating the health risk of multiple pesticide residues in Chaenomeles speciosa and strengthening the risk management of pesticide residues in Chinese medicines.