不同LED光质调控微藻单细胞碳分配机制研究

微藻是类胡萝卜素、油脂等高附加值产物的重要来源，应用前景广阔。微藻在积累高值组分同时常伴随大量廉价有机物如碳水化合物、蛋白质的合成，这四类有机物是微藻胞内碳存在的主要形式，各自合成存在相互影响、共同竞争碳源底物现象。由于对微藻胞内碳分配代谢机制不清，导致微藻高值组分生产不够稳定、产率不高，成为影响微藻产业化发展的主要难点之一。本项目突破现有研究均以藻群为对象，存在细胞异质性被忽略、分析步骤复杂繁琐造成的样品损失和结论失真等问题，创新性地将显微拉曼光谱技术与单细胞生化分析技术有机结合，利用显微拉曼光谱仪实现单细胞内四类有机物成像分析，利用单细胞仪实现痕量碳代谢中间产物单分子特异性快速检测，两者优势互补，可有效追踪不同LED光质下微藻胞内碳分流、储运及互作关系，揭示微藻单细胞碳分配机制，并最终获得微藻单细胞高效优质生产的优化光质方案。以期通过提高微藻个体细胞产能，从根本上实现藻群的稳定高产。

Microalgae, an important source of high-value organic compounds such as carotenoids and lipids, have broad application prospects. Large amounts of low-value organic compounds (e.g. carbohydrates, proteins) are formed during the accumulation of high-value products in microalgae. These four kinds of substances are the main forms of intracellular carbon in microalgae, and their anabolisms affect each other and compete for carbon substrate. Little is known about the mechanisms determining the synthesis and metabolism of organic products and carbon partitioning in microalgae, which leads to the unstable and low yield of high-value products of microalgae. That is one of the main difficulties that influence the development of microalgae industrialization. This project breaks through the limitations of the present studies, which all focus on microalgal population, have problems such as ignorance of cell heterogeneity and the loss of samples due to the complexity of analysis steps, and thereby cause the distortion of conclusions. By using the micro-Raman spectroscopy which is suitable for the imaging analysis of organic products in single microalgal cells, combining with the single cell analyzer which is an ideal means in detection of trace intermediates of carbon metabolism, to study the distribution, storage, transportation and interaction of microalgal intracellular carbon under different LED light quality, to reveal the mechanisms of intracellular carbon partition of microalgae, thereby to develop an optimized artificial supplementary lighting for high efficiency and quality production of single microalgal cells. The project is expected to achieve stable and high yield of microalgal population by improving individual cell productivity.