基于NALT免疫功能研究艾叶油对猫疱疹病毒感染的干预作用及分子机制

猫疱疹病毒通过眼、鼻黏膜侵入机体，引起猫病毒性鼻气管炎，严重威胁着猫的生命健康。前期研究发现艾叶油可增强猫呼吸道黏膜免疫机能，可有效防治该病，但其作用机制尚不明确。鼻相关淋巴组织是上呼吸道黏膜免疫系统的重要组成部分，是抗击猫疱疹病毒的第一道防线。因此，本项目以猫鼻相关淋巴组织黏膜免疫机能变化为出发点，首先研究艾叶油的体外抗FHV-1作用；然后构建猫疱疹病毒感染模型，通过临床症状评分、ELISA、Q-PCR、HE染色等技术评价艾叶油对感染疱疹病毒猫的鼻相关淋巴组织的免疫机能调控作用；最后通过鼻相关淋巴组织分离、流式细胞术分析、细胞因子检测等方法，研究艾叶油调节鼻相关淋巴组织黏膜免疫信号从而抵御病毒侵袭的分子机制。本研究将为艾叶油鼻腔给药防治猫疱疹病毒感染提供依据，阐释艾叶油对猫呼吸道黏膜免疫机能的调控机制。

Feline herpes virus invades the body through the eyes and nasal mucosa, causing feline viral rhinotracheitis, which seriously threatens the life and health of cats. Previous studies have found that artemisia argyi oil has the effect of enhancing mucosal immunity and can prevent and treat the disease effectively, but the mechanism is not yet clear. Nasal-associated lymphoid tissue is an important part of the upper respiratory tract mucosal immune system and is the first line of defense against feline herpes virus. Therefore, this project based on the mucosal immune signaling pathway of nasal-associated lymphoid tissue, firstly, the in vitro anti-FHV-1 effect of artemisia argyi oil will be studied;then, based on the establishment of feline herpes virus infection disease model, the clinical symptoms score, ELISA, Q-PCR, HE staining and other techniques will be used to evaluate the immunoregulatory effect of artemisia argyi oil on nasal-associated lymphoid tissue; finally through the methods of nasal associated lymphoid tissue isolation, flow cytometry analysis and cytokine detection, the antiviral molecular mechanism of artemisia argyi oil regulating mucosal immune signaling in nasal associated lymphoid tissues will be studied. This study will provide evidence for the nasal administration of artemisia argyi oil to prevent and treat feline herpes virus infection, and elucidate the regulatory mechanism of artemisia argyi oil on the immune function of the cat's respiratory mucosa.