肌源性神经营养因子在急性共同性内斜视中眼外肌神经支配的相关研究

急性共同性内斜视（AACE）是一种起病急骤的特殊共同性内斜视，伴复视，发病机制尚不明确。近年发现，AACE大多无颅内和全身器质性疾病，与近距离阅读增多存在正相关性，可能与眼外肌异常神经支配有关，但缺乏分子水平的研究证据。肌源性神经营养因子中的胶质细胞源性神经营养因子（GDNF）和胰岛素样生长因子-1（IGF-1）是眼外肌发育和重塑的重要因子，参与眼外肌神经支配，影响眼外肌收缩。本课题旨在探索AACE眼外肌发生异常神经支配与GDNF和IGF-1在眼外肌短期内含量异常有关的分子生物学水平证据。拟研究（1）AACE眼外肌是否发生异常神经支配；（2）验证AACE眼外肌异常神经支配和GDNF、IGF-1含量异常相关性；（3）动物模型验证短期降低GDNF和IGF-1在眼外肌含量，是否引起眼外肌异常神经支配。本课题为探索ACCE的分子生物学证据，研发预防和治疗相关药物提供科学依据，满足急性发病人群需求。

Acute concomitant esotropia (AACE) is a special concomitant esotropia with a sudden onset, accompanied by diplopia, and its pathogenesis is not clear. In recent years, it has been found that most of AACE has no intracranial and systemic organic diseases, and is positively correlated with increased close reading, which may be related to abnormal innervation of extraocular muscles, but there is a lack of evidence at the molecular level. Glial cell-derived neurotrophic factor (GDNF) and insulin-like growth factor-1 (IGF-1) in muscle-derived neurotrophic factor are important factors for the development and remodeling of extraocular muscles. They are involved in the innervation of extraocular muscles, and affect the contraction of extraocular muscles. This project aims to explore the molecular biological evidence that the abnormal innervation of AACE extraocular muscles is related to the short-term abnormal content of GDNF and IGF-1 in extraocular muscles. To study (1) whether abnormal innervation of AACE extraocular muscles occurs; (2) whether the correlation exists between abnormal innervation of AACE extraocular muscles and abnormal content of GDNF and IGF-1; (3) whether short-term reduction of GDNF and IGF-1 in extraocular muscles, may cause abnormal innervation of extraocular muscles in animal models. This project provides scientific basis for exploring the molecular biology evidence of AACE prevention and therapy, and will meet the needs of AACE patients.