烙灸激活Wnt信号通路促进脊髓损伤大鼠内源性神经干细胞增殖分化研究

脊髓损伤是医学界面临难题。本人采用烙灸治疗脊髓损伤能提高患者肌力，但其治疗机制尚未完全阐明。前期采用基因芯片技术研究烙灸对脊髓损伤大鼠基因表达谱变化，筛查出Wnt-1、Wnt-2b等基因与Wnt信号通路相关，文献证实脊髓损伤与Wnt信号通路相关。实验显示烙灸上调脊髓损伤大鼠Wnt－1、β－catenin蛋白表达，激活Wnt信号通路，而研究表明Wnt信号通路被激活又能促进内源性神经干细胞增殖分化为神经元，填充受损区域，促进神经功能恢复。由此推测烙灸激活脊髓损伤大鼠Wnt信号通路，促进其内源性神经干细胞增殖分化为神经元及少突胶质细胞，同时抑制分化为星形胶质细胞，以达到神经细胞修复再生。为验证假说，拟复制Allen's脊髓损伤大鼠模型，从大鼠离体和在体实验入手，采用多种分子生物学技术，抑制Wnt信号通路揭示烙灸对脊髓损伤大鼠内源性神经干增殖分化作用机制，为脊髓损伤后神经细胞修复再生提供实验基础。

Spinal cord injury has always been a difficult problem in medical field.My treatment of spinal cord injury with lao moxibustion can improve the muscle strength of patients, but the mechanism of treating spinal cord injury has not been fully elucidated.Gene chip technology was used to study gene expression profile changes of spinal cord injury rats in the early stage. Screening found that genes such as Wnt-1 and Wnt-2b are related to Wnt signaling pathways. Literature studies have also confirmed that spinal cord injuries are related to Wnt signaling pathways.Experiments showed that lao moxibustion could up-regulate the expression of Wnt-1 and β－catenin proteins in rats with spinal cord injury, activating the Wnt signaling pathway, while studies showed that the activation of the Wnt signaling pathway could promote the proliferation and differentiation of endogenous neural stem cells into neurons, fill in the damaged areas and promote the recovery of nerve function.It is speculated that the moxibustion activates the Wnt signaling pathway in spinal cord injured rats, promotes the proliferation and differentiation of endogenous neural stem cells into neurons and oligodendrocytes, and inhibits the differentiation into astrocytes to achieve nerve cell repair and regeneration.To test the hypothesis, Allen's rat model of spinal cord injury is copied. Starting with in vitro and in vivo experiments in rats, various molecular biological techniques are used to suppress Wnt signaling pathway to reveal the mechanism of the effect of lao moxibustion on the proliferation and differentiation of endogenous neural stem cells in spinal cord injury rats, so as to provide experimental basis for the repair and regeneration of nerve cells after spinal cord injury.