WRKY转录因子协同茉莉酸信号调控三七对茄腐镰刀菌抗性的分子机制研究

三七是中国特有的名贵中药材。由于长期留种栽培和阴湿的生长环境，三七在种植过程中病虫害多发。根腐病是三七的主要病害，前期研究发现茉莉酸信号途径正调控三七对根腐病菌茄腐镰刀菌的抗性，并且大量WRKY转录因子基因响应茉莉酸信号。WRKY转录因子调控植物的转录重编程过程，对植物防御反应的启动至关重要，然而，WRKY转录因子协同茉莉酸信号参与三七防御茄腐镰刀菌的分子机制知之甚少。为此，本项目从三七中分离协同茉莉酸信号参与茄腐镰刀菌防卫反应的WRKY基因；分析其基因表达谱和亚细胞定位；验证WRKY转录因子结合W盒以及激活转录的特性；研究其与茉莉酸响应抗病基因的相互作用；明确WRKY基因的功能；揭示三七与根腐病菌互作过程中WRKY转录因子与茉莉酸信号协同调控的分子网络。项目的开展不仅有助于深入认识茉莉酸信号介导的三七与根腐病菌分子互作过程的转录调控机制，还能推动三七的抗病遗传育种研究。

Abstract.Sanqi (Panax notoginseng) is a precious Chinese herbal medicine. but P. notoginseng is susceptible to a lot of diseases and insect pests due to long term reserve seed for planting and damp growth environment. The root rot is the most serious disease, and our previous study showed that the jasmonic acid signaling pathway positively modulates the resistance of P. notoginseng to the root rot pathogen Fusarium solani, moreover, a series of WRKY transcription factor genes respond to the jasmonic acid signaling. The WRKY transcription factors regulate the transcription reprogramming in plant cells, that is very important for the initiation of host defense response. However, the synergistical regulation mechanism of jasmonic acid signaling and WRKY transcription factors in P. notoginseng during response to F. solani is not clear now. Therefore, the WRKY genes responding to the jasmonic acid signaling and F. solani infection will be isolated from P. notoginseng in this study, and their expression patterns under normal conditions and after inoculation with F. solani will be analyzed. The subcellular localization, specific binding of W-box, and transcription activation of P. notoginseng WRKYs will be further studied. In addition, the transcriptional regulation of the resistance related genes by WRKY transcription factors will be researched. Lastly, the gene function of some P. notoginseng WRKY genes and the synergistically regulated molecular net by WRKY transcription factors and the jasmonic acid signaling during response to F. solani infection will be revealed. The results of this study would help to further understand the transcriptional regulation mechanism of WRKYs coordinating with the jasmonic acid signaling during interaction between the P. notoginseng and F. solani. Additionally, the P. notoginseng genetic breeding research should be promoted based on the data of this study.