浙江省农作物重要媒介昆虫携带的病毒风险评估及其共生病毒影响植物病毒传播的机制

媒介昆虫传播的植物病毒病害的间歇性爆发，已成为限制浙江省农业安全生产的重要制约因素之一。当前植物病毒研究对象主要是田间危害严重的已知植物病毒，而对于媒介昆虫中具有潜在风险的植物病毒及其共生病毒在植物病毒传播中的作用鲜有报道。目前自然界中绝大多数病毒未知，宏基因组新一代测序技术（mNGS）为新病毒高通量快速鉴定提供了全新的研究方法，并在近几年重大公共卫生事件的病原鉴定中发挥了关键作用。本申请项目拟综合运用mNGS、分子生物学、病毒学等方法全面鉴定和分析浙江省重要媒介昆虫中的病毒多样性，利用反向病原学方法对媒介昆虫中新鉴定的植物病毒（或已知植物病毒的新传毒媒介）的爆发风险进行科学评估，并进一步解析媒介昆虫中的共生病毒对植物病毒传播的影响及其分子机制。项目研究结果将完善和推进目前昆虫和植物病毒的分类系统，有助于植物病毒病害爆发前及爆发初期的准确预警和正确防控，并为浙江省农业绿色防控提供科学依据。

Intermittent outbreaks of plant virus diseases transmitted by insect vectors have become one of the important constraints for agricultural safety in Zhejiang Province. The current research of plant virus disease is mainly focused on known important plant viruses, and there is limited report for the evaluation on the potentially risk of newly emerged plant viruses and insect specific virus on the vector competence of the transmitted plant viruses. The vast majority of viruses in nature are still unknown at present, and metagenomic of the next generation sequencing technology (mNGS) provides a high-throughput method for the rapid identification of new viruses, which has played an essential role for rapid pathogens identification in recent public health events. This project intends to combine the methods of mNGS, molecular biology and virology to comprehensively identify and analyze the viral diversity of agricultural important insect vectors in Zhejiang province. And to assess the risk of newly identified plant viruses in insect vectors scientifically using reverse microbial etiology method. Finally, the effects and molecular mechanism of insect specific virus on the vector competence of the transmitted plant viruses will further be investigated. The results of this project will improve the current taxonomy status of the insect and plant viruses, and help to control the outbreak of newly emerged plant virus diseases in early stage, which will contribute to the agricultural green prevention and control in Zhejiang province.