森林片段化如何影响物种种群空间遗传结构是保育遗传学的重要科学问题。附生兰科植物对生境变化敏感，其花粉扩散过程易受干扰。本研究以西双版纳橡胶种植导致的森林片段化为背景，选择传粉系统特化的附生兰科植物禾叶贝母兰为材料，以自然保护区连续森林为对照，调查片段化斑块森林中禾叶贝母兰的种群结构、传粉昆虫的丰富度、花粉移出率与沉降率及繁殖成功，并利用nDNASSR对母系半同胞家系进行父本分析追踪花粉来源，阐明森林片段化对附生兰科植物花粉散布的影响；利用nDNASSR与cpDNASSR 分析其不同世代种群空间遗传结构，评估森林片段化对附生兰科植物种群空间遗传结构的影响，并结合前面的调查结果，分析其中花粉散布的相对贡献；再综合与斑块森林的形成时间、面积大小、形状、隔离程度及地理与环境等参数的回归分析结果，进一步探索森林片段化遗传效应背后的调控因素。研究结果将为该地区生态恢复与附生兰科物种保护提供理支持。

It is an important scientific subject for conservation genetics to understand the effects of habitat fragmentation on population genetic diversity and spatial genetic structure of plants.The epiphytic orchids are vunerable to forest fragmentation, and particularly, their pollen dispersal patterns are easily disrupted in fragmented habitats, which will further affect the population spatial genetic structure.The natural forests have become severely fragmented by the rubber tree plantation in Xishuangbanna region. In this study, the population structure, the abundances of pollinator and the frequencies of their visiting flowers, the rates of pollen removal and deposition, and reproductive success of Coelogyne viscose, an epiphytic orchid with specialized pollination system,will be investigated in a forest fragmentation plot network in Xishuangbanna region.And nuclear DNA (nDNA) SSR marker will be used to track the paternal origin of the maternal half-sib progenies and to characterize the pollen dispersal patterns.These findings, combinated with the above results of investigation, will show the effects of forest fragmentation on the pollen dispersal of this epiphytic orchid.Then,the genetic diversity and spatical strutures across three generations of these populations will be examined with both nDNA SSR and chloroplast DNA (cpDNA) SSR markers to identify the effects of forest fragmentation on spatial genetic strutures,and the relative contribution of pollen dispersal to the genetic structures. Also, multi linear regressions will be taken between among the molecular results described aboved and the variables of fragmented forests, incuding size, shape, duration, isolation and geographic and enviromental indecies,to explore which factors of forest fragmented habitat are mainly responsible for the genetic affects of forest fragmentation.This research will provide the theoretical foundation for the ecological restoration in Xishuangbanna region and spcieses conservation of the endangered epiphytic orchids.