毕氏肠微孢子虫遗传演化规律及其优势基因型D的传播动力学分析

针对缺乏人兽共患毕氏肠微孢子虫在我国家禽中的分布特征及流行传播规律的理论需求，围绕毕氏肠微孢子虫遗传演化趋势促进其传播动力学的阐明这一科学问题，采用遗传学、系统进化学、生物信息学、分子流行病学及生物学的技术与方法，以毕氏肠微孢子虫优势基因型D为研究对象，重点研究：1）构建已报道的毕氏肠微孢子虫基因型及其流行信息网络云数据库平台；2）采用距离法、最大似然估计和贝叶斯推理三种算法和计算机编程及生物信息学技术，对已报道的近700个基因型进行系统遗传进化分析；结合流行信息，阐明优势基因型D在我国及全球不同宿主和地域范围的流行传播规律；3）开展毕氏肠微孢子虫在中国家禽的分子流行调查，了解其在我国家禽中分布情况，以及病原、宿主和环境的相互作用，初步解析其流行传播的“三间”分布规律。研究结果将有助于阐明毕氏肠微孢子虫遗传演化特征和流行传播规律，为有效防控毕氏肠微孢子虫在我国家禽中感染和爆发提供理论依据。

Enterocytozoon bieneusi is the most common microsporidian protozoa that infect both humans and animals, causing diarrhea and systemic diseases. Nearly 700 E.bieneusi genotypes have been characterized until now, however, there has been no systematic epidemiological and phylogenetic evolutionary analyses including all E.bieneusi genotypes and their epidemiological data internationally. In order to fix the gap, the applicant uses the previous established data sets of E. bieneusi genotypes and epidemiological information, in terms of this scientific issue -phylogenetic evolutionary analyses can facilitate the transmission dynamics, using genomics, genetics, phylogenetics, molecular detection and bioinformatics, the aims of this project are： 1）to establish a database of all global E. bieneusi genotypic and epidemiological； 2）to conduct phylogenetic analyses of all E. bieneusi genotypes using three（Distance, Maximum likelihood and Bayesian inference）phylogenetic methods and study the transmission dynamics of predominant genotype D in China and around the world； 3) conduct molecular identifications of E. bieneusi in chickens from representative locations in China, analyze the co-interaction of pathogen, hosts and environment, and the patterns of transmission dynamics. The results would illustrate the patterns and trends of E. bieneusi transmission and access zoonotic potential of new genotypes, both of which would eventually assist in the prevention and control of outbreaks of Enterocytozoonosis in humans and animals worldwide.