基于MYB10AG-insert/MYB10nomal竞争抑制调控栽培草莓果实色泽的分子机制解析

白果草莓由于市场量少，具有特殊的风味，具有很高的市场价值。白果草莓育种已经成为草莓育种的一个新方向。解析草莓果实中花色苷形成机理有助于实现定向育种，提高育种效率。本项目利用‘红颊’和白肉突变材料‘小白’为试材，发现杂合突变(MYB10AG-insert/MYB10normal)与白肉突变有关，拟开展MYB10AG-insert/MYB10nomal竞争抑制调控栽培草莓果实色泽形成的分子机制。首先对两份材料开展转录组测序和黄酮差异物质分析获得关键花色苷结构基因；随后通过转基因验证MYB10AG-insert基因功能；通过蛋白质和蛋白质、蛋白质和DNA互作的方法，验证MYB10nomal是否能形成MBW复合体调控下游关键结构基因表达，以及MYB10AG-insert对这一过程是否存在竞争性抑制。研究结果不仅有助于解析栽培草莓果实颜色形成机理，同时对草莓果实颜色育种提供分子标记。

Due to the lack of supply on the market and special flavor, the white fruit strawberry varieties have great market value. Therefore, white fruit strawberry breeding has become a new direction. Understanding of the underlying molecular mechanisms of anthocyanin accumulation in strawberry fruit is helpful to realize directional breeding of strawberry fruit color and improve breeding efficiency. Using‘Benihoppe’ and its white flesh mutation ‘Xiaobai’, heterozygous frameshift mutation （MYB10nomal/MYB10AG-insert）was found to be associated with white flesh. This study will use these two materials to analysis the molecular mechanism of MYB10AG-insert/MYB10nomal competitive inhibition in regulation of fruit color of strawberry. Firstly, transcriptome sequencing and detection of flavonoid contents were carried out in the two materials to identify the key differential expression of anthocyanin pathway genes. Subsequently，MYB10AG-insert gene was overexpressed by transgenic technology to verify gene function in color formation of strawberry flesh. Then, we use the research method of protein-protein, protein-DNA interaction to analyzed whether MYB10AG-insert was competitive with MYB10nomal in form MBW complex, which regulate the expression of ant genes. The research result will not only contribute to further understanding of the strawberry fruit color formation mechanism, but also provide the molecular marker basis for the future breeding of strawberry varieties with different colors.