杨树生长调节因子GRF11b调控分枝发育的机理研究

基于前期杨树分枝发育QTL定位和表达结果表明，PtGRF11b可能参与杨树的分枝发育调控，但其调控机理还不清楚。PtGRF11b是生长调节因子（Growth-Regulating Factor, GRF）基因家族成员，编码一类植物特有的转录因子，在植物生长发育过程中发挥着重要调控作用。因此，本项目拟围绕PtGRF11b调控杨树分枝发育的分子机理这一科学问题，采用细胞生物学、分子生物学、生物化学等技术与办法，以白杨1号为对象，重点研究：1)PtGRF11b在分枝发育中的组织表达模式；2)创制mPtGRF11b-OE和PtGRF11b-KO等转基因材料，分析其在分枝发育中的生物学功能；3)通过RNA-seq和ChIP-seq等分析PtGRF11b调控的下游靶基因，阐明PtGRF11b调控分枝发育的分子机制。预期研究结果将丰富木本植物分枝发育的理论研究，为林木优质遗传改良提供理论基础与候选基因。

Based on the QTL and expression results of poplar branch development, PtGRF11b may be involved in the regulation of poplar branch development, but the regulation mechanism is still unclear. PtGRF11b is a member of the Growth-Regulating Factor (GRF) gene family. It encodes a type of plant-specific transcription factor and plays an important regulatory role in the growth and development of plants. Focusing on the molecular mechanism of GRFs involved in branching development of woody plants, this project adopts cell biology and molecular biology techniques, with Populus Leucopyramidalis 1 as the object, and focuses on the following aspects: 1) the expression pattern of PtGRF11b was determined by GUS staining and in situ hybridization; 2) The biological function of PtGRF11b in branching development was analyzed by creating transgenic materials with mPtGRF11b-OE (with mutations in the miR396 target site) and PtGRF11b-KO(PtGRF11b CRISP knock out); 3) The downstream regulatory target genes of PtGRF11b were analyzed by RNA-seq and ChIP-seq to elucidate the molecular mechanism of PtGRF11b regulating branching development. It is expected that the research results will enrich the theoretical research on branching development of woody plants and provide theoretical basis and candidate genes for genetic improvement of high quality trees.