MicroRNA在微囊藻毒素LR暴露诱导的犊牛睾丸Sertoli细胞炎症反应中的调控机制研究

微囊藻毒素LR（MC-LR）对人畜生殖健康造成危害，尤其对大型养殖业及游牧业造成的经济损失不可估量。最近的研究均在试图挽救由MC-LR暴露导致的Sertoli细胞损伤，但缺乏有效办法。由此推测MC-LR暴露诱导的Sertoli细胞相关分子机制仍不完善。因此，通过测量Sertoli细胞中miRNA改变来研究MC-LR潜在分子机制是打破这一瓶颈有效解决办法。我们前期的研究揭示MC-LR暴露可引起牛睾丸Sertoli细胞免疫反应，推测miRNA可能在Sertoli细胞免疫反应中参与调控作用。因此，本项目以MC-LR暴露引发Sertoli细胞炎症反应为媒介，通过miRNA微阵列分析，阐明miRNA在Sertoli细胞炎症反应中的功能及其调控机制，为MC-LR诱导的Sertoli细胞毒性分子机制提供新的表观遗传基础，同时为后续筛选治疗Sertoli细胞炎症损伤的药物和解决办法提供新思路。

Microcystin-LR (MC-LR) causes harm to human and animal reproductive health, especially the economic losses caused by large-scale breeding and nomadic industries. Recent studies are trying to rescue the Sertoli cell damage caused by MC-LR exposure, but there is no effective way. It is speculated that the related molecular mechanism of Sertoli cells induced by MC-LR exposure is still imperfect. Therefore, studying the potential molecular mechanism of MC-LR by measuring the changes of MicroRNA (miRNA) in Sertoli cells is an effective solution to break this bottleneck. Our previous studies revealed that MC-LR exposure can cause the immune response of bovine testicular Sertoli cells. It is speculated that miRNA may participate in the regulation of the Sertoli cell immune response induced by MC-LR exposure. Therefore, this project aims to elucidate the function and regulatory mechanism of miRNA in Sertoli cell inflammatory response induced by MC-LR through bioinformatics analysis, so as to provide a new epigenetic basis for the molecular mechanism of MC-LR induced Sertoli cytotoxicity. At the same time, it provides new ideas for the follow-up screening of drugs and solutions for the treatment of Sertoli cell inflammatory injury.