功能化球形核酸用于胶质瘤缺氧相关信号通路分子的传感分析及肿瘤治疗研究

研究表明胶质瘤的高度迁移性、浸润性、耐药性均与胶质瘤缺氧密切相关。尽管最近几年针对于肿瘤缺氧的研究蓬勃发展，然而现有工作多聚焦于新型缺氧响应基团的开发，在分子生物学层面上深入研究胶质瘤缺氧相关信号通路分子的表达及作用机制仍然是目前的热点问题之一。本项目结合已有的工作基础，拟利用球形核酸分子探针作为研究工具，发展新的传感机理及信号转导方法实现胶质瘤缺氧及其相关信号通路分子的高灵敏分析；同时拟构建多响应智能球形核酸探针传感平台，实时、原位调控缺氧相关信号通路分子的生理功能，这将有助于深入研究胶质瘤缺氧与其浸润性、耐药性间的相互关系；并在此基础上发展新型高效的胶质瘤治疗方法，为胶质瘤的高效治疗及良好提供预后新的思路和手段。

Studies have demonstrated that the high mobility, infiltration and drug resistance of glioma were closely related to the hypoxic. Although the current study for tumor hypoxia has developed rapidly in recent years, the existing researches still focus on the development of new hypoxia responsive groups, the study on the molecular biological changes of glioma cells in hypoxia is still in the ascendant. In this project, we proposed to use spherical nucleic acid to develop new sensing mechanism and signal transduction method to realize the high-sensitivity analysis of biological molecules in the hypoxic microenvironment of glioma and its related signal pathway. Meanwhile, multi-response intelligent spherical nucleic acid probe sensing platforms were proposed for regulating the physiological function of hypoxia related signal pathway molecules in real time and in situ, which will help to reveal the relationship between the hypoxia microenvironment of glioma and its invasion and drug resistance. Furthermore, we will develop new and efficient glioma treatment methods, and provide new ideas and means for high-efficiency treatment and good prognosis for glioma.