慢性中耳炎是耳鼻咽喉科难治性疾病之一，了解病原菌及其细菌生物膜在自然免疫中的作用,对于揭示不同病原菌及细菌生物膜在慢性中耳炎的主要致病机制研究具有重要意义。本课题组前期研究已经证实细菌生物膜在慢性中耳炎中的存在，而且与常规细菌培养之间存在差异。但是形成细菌生物膜的病原菌种类，以及细菌生物膜形成在慢性中耳炎自然免疫中的可能作用机制，国内外报道甚少。我们推测不同种类细菌形成的细菌生物膜，可能导致中耳粘膜产生不同的自然免疫应答。为证实一假说，我们采用细菌培养、PCR技术、电镜检查、免疫组化等技术，从细菌-细菌生物膜-细胞三个层面，探讨慢性化脓性中耳炎病原菌形成细菌生物膜情况、细菌生物膜形成对中耳粘膜信号传导通路的影响。本课题将从新的视觉研究CSOM 发病的分子机制，并为 CSOM的防治提供新途径。

Chronic otitis media is one of the refractory diseases in otolaryngology department.To know pathogen and the role of its bacterial biofilm in natural immune,there is of great significance to reveal the main pathogenic mechanism of chronic otitis media about different pathogenic bacteria and bacterial biofilm .Our previous studies have demonstrated bacterial biofilms in chronic otitis media, and there was the differences between conventional bacterial culture and bacterial biofilm.But the pathogenic bacteria species of bacterial biofilm formation, and the main pathogenic mechanismof bacteria biofilm formation in chronic otitis mediathe ,there is lack of research reported at home and abroad.We hypothesized that due to different species bacteria biofilm formation, may lead to produce different natural immune response in middle ear mucosa .In order to verify the hypothesis, we use the bacterial culture, PCR technique, electron microscopy (SEM) examination and immunohistochemical technique, from bacteria cells - bacterial biofilm - cell, to explore the bacteria biofilm situation of pathogen in chronic suppurative otitis media ,the influence of bacterial biofilm formation of middle ear mucosa on signaling pathways.This topic will be formed a new visual research about CSOM cmechanism of the disease, and offers a new approach for prevention of CSOM.