多胺与脱落酸协同调控草莓节水和果实品质形成的机制研究

设施草莓已成为我国特色果树产业。草莓根系浅、需水量大且对土壤水分胁迫极为敏感。在水资源匮乏的北方地区，开展设施草莓节水和果实品质提升的理论技术研究具有重要意义。尽管前期研究在一定程度上证实多胺（Polyamines，PAs）与脱落酸（Abscisic acid，ABA）在草莓节水和果实品质的形成发挥着重要作用，但其协同调控节水潜力及品质改善的机制尚不清楚。因此，本项目借助氮稳定同位素示踪法和草莓克隆植株特性，通过生态学、植物生理学和分子生物学方法，以水-肥-生长调节剂综合调控为切入点，分析干旱胁迫早期草莓叶片与果实中PAs、ABA水平动态变化及转运特征，揭示PAs、ABA对草莓生长及果实品质影响的作用关系和地位，解析PAs与ABA对草莓耐旱性及果实品质形成的协同调控效应，研究草莓根、叶、果器官内源PAs与ABA诱导累积调控技术，最终为草莓节水栽培和果实品质改善提供理论依据。

Greenhouse strawberry, with off-season supply of fresh fruit in winter/spring and high economic effectiveness, has become distinct fruit planting in China. Strawberry roots are shallow, require a large amount of water for growth, and are very sensitive to soil water stress. It is required for study of mechanisms underlying strawberry water saving and fruit quality improvement, that is great scientific significance and application prospect in North China with water resources seriously deficient. Although the pre-researches, to some extents, have confirmed that polyamines (PAs) and abscisic acid (ABA) play an important role in strawberry water-saving and fruit quality formation, the coordinated regulation mechanism of both in plant water-saving potential and fruit quality improvement is not clear. In this project, nitrogen stable isotope tracing method and strawberry clonal plant are used to explore by a combination of ecological, physiological and molecular methods. Based on water-fertilizer-growth regulator integration system, we first analyze dynamic changes and transport characteristics of PAs and ABA in strawberry leaves and fruits at the early stage of drought stress, uncovering the role and extent of PAs and ABA on strawberry growth and fruit quality, determining the synergistic regulation effect of PAs and ABA on strawberry drought tolerance and fruit quality formation. Finally, we further explore the regulation measures to induce endogenous PAs and ABA cumulation in strawberry roots, leaves and fruits. Our results will provide theoretical and technique supporting for strawberry water-saving and fruit quality improvement.