基于小分子药物/BMP-2修饰的原位引导骨再生材料的构建及其协同促进成骨机理研究

基于“功能性生物材料和生长因子”构建引导骨组织再生材料是提升现有骨修复材料活性的理想途径。BMP-2是已知成骨活性最强的生长因子之一，也是构建原位引导骨再生材料的重要因子。研究发现部分小分子药物，如地塞米松、维生素C等，对BMP-2的诱导成骨活性有显著的影响。为此，本项目将紧紧围绕“小分子药物与BMP-2协同诱导成骨”这一研究目标，力图通过新型可快速降解枝状介孔硅载体（D-MSNs）的引入，实现BMP-2和小分子药物的时序控释，维持蛋白活性，降低BMP-2的使用量。并进一步通过3D生物打印技术，制备含有小分子药物/BMP-2/D-MSNs的三维多孔支架，构建高活性人工骨材料。以此为基础，建立小分子药物/BMP-2/D-MSNs促进骨组织快速修复的基本理论。

The construction of guided bone tissue regeneration (GBTR) materials based on "functional biomaterials and growth factors" is an ideal way to enhance the activity of existing bone repair materials. BMP-2 is known to have the strongest osteogenic activity, and is also an important factor in the construction of in situ guided bone regeneration materials. It is found that some small drugs, such as dexamethasone, vitamin C, have a significant impact on the osteogenic activity of BMP-2. Therefore, the project will focus on the research goal of “small drugs and BMP-2 induced osteogenesis synergistically”, using a kind of rapid-degradation dendritic mesoporous silica nanoparticles (D-MSNs) as carriers, in order to maintain the bioactivity of protein and controlled release of the drug/protein. With the synergistic effect, we will achieve the regulation of cell behavior and reduce the usage of BMP-2. And then, with 3D printing technology, a kind of scaffold of high osteogenic activity containing small drug/BMP-2/D-MSNs will be developed. Based on these studies, the theory about the enhancement of rapid bone tissue repair with small drug/BMP-2/D-MSNs will be summarized and revealed.