StMKK1调控马铃薯对晚疫病菌免疫应答的机理研究

MAPK级联系统在植物免疫中发挥重要作用，本研究前期发现马铃薯MAPK类基因StMKK1负调控本氏烟草对晚疫病菌的免疫反应，且激酶活性与其负调控植物免疫相关。但是StMKK1通过何种调控网络和分子机制影响马铃薯对晚疫病菌的免疫应答有待进一步解析。研究前期筛选并验证了酪氨酸磷酸酶StPTP、MAPK类蛋白StMPKa和StMPK4为StMKK1的互作蛋白。本研究拟通过过表达和敲除马铃薯StMKK1基因研究其调控马铃薯对晚疫病菌免疫应答的作用；通过鉴定StPTP、StMPKa和StMPK4调控马铃薯免疫的功能，研究StMKK1与StPTP、StMPKa、StMPK4之间的互作，分析磷酸化、去磷酸化以及蛋白稳定性与蛋白互作的关系，明确StMKK1的调控网络，以期解析StMKK1调控马铃薯免疫的分子机制。研究结果将为马铃薯抗病育种提供新型抗病基因资源，为改良和培育马铃薯抗病品种提供理论指导。

MAPK proteins play important roles in plant immunity. Our previous research showed that potato StMKK1 gene negatively regulates Nicotiana benthamiana defense response to the late blight disease, and the kinase activity is responsible for its negative regulation of immunity. However, the mechanism and the regulation network that StMKK1 uses to modulate potato immunity is not known. Previously, we performed a yeast two hybrid screening to identify StMKK1-interacting proteins, and we have confirmed that the Protein Tyrosine Phosphatase StPTP, the MAP kinase proteins StMPKa and StMPK4 interact with StMKK1. This study, we plan to overexpress and knock out StMKK1 in potato to study the role of StMKK1 in regulation of potato immunity to the late blight disease; to investigate the role of StPTP, StMPKa and StMPK4 in potato immunity; to study the relationships between StMKK1 and StPTP, StMPKa, StMPK4 in protein interaction, phosphorylation, dephosphorylation, protein stabilities to clarify the regulation network of StMKK1, in order to reveal the mechanism of StMKK1 in regulation of plant immunity. The results from this research will provide new disease resistance gene resources for potato resistant breeding, and provide theoretical guidance for improving and cultivating potato disease resistant cultivars.